Structural and biochemical dissection of photorespiration in hybrids differing in genome constitution between Diplotaxis tenuifolia (C3-C4) and radish (C3)

Osamu Ueno, Sang Woo Bang, Yoshiharu Wada, Ayumu Kondo, Kuni Ishihara, Yukio Kaneko, Yasuo Matsuzawa

研究成果: ジャーナルへの寄稿記事

27 引用 (Scopus)

抄録

We compared the structural, biochemical, and physiological characteristics involved in photorespiration of intergeneric hybrids differing in genome constitution (DtDtR, DtDtRR, and DtRR) between the C3-C4 intermediate species Diplotaxis tenuifolia (DtDt) and the C3 species radish (Raphanus sativus; RR). The bundle sheath (BS) cells in D. tenuifolia included many centripetally located chloroplasts and mitochondria, but those of radish had only a few chloroplasts and mitochondria. In the hybrids, the numbers of chloroplasts and mitochondria, the ratio of centripetally located organdies to total organelles, and the mitochondrial size in the BS cells increased with an increase in the constitution ratio of the Dt:R genome. The P-protein of glycine decarboxylase (GDC) was confined to the BS mitochondria in D. tenuifolia, whereas in radish, it accumulated more densely in the mesophyll than in the BS mitochondria. In the hybrids, more intense accumulation of GDC in the BS relative to the mesophyll mitochondria occurred with an increase in the Dt:R ratio. These structural and biochemical features in the hybrids were reflected in the gas exchange characteristics of leaves, such as the CO2 compensation point. Our data indicate that the leaf structure, the intercellular pattern of GDC expression, and the gas exchange characteristics of C3-C4 intermediate photosynthesis are inherited in the hybrids depending on the constitution ratio of the parent genomes. Our findings also demonstrate that the apparent reduced photorespiration in C3-C4 intermediate plants is mainly due to the structural differentiation of mitochondria and chloroplasts in the BS cells combined with the BS-dominant expression of GDC.

元の言語英語
ページ(範囲)1550-1559
ページ数10
ジャーナルPlant physiology
132
発行部数3
DOI
出版物ステータス出版済み - 7 1 2003

Fingerprint

Diplotaxis tenuifolia
Raphanus
photorespiration
Constitution and Bylaws
radishes
Glycine Dehydrogenase (Decarboxylating)
Dissection
Mitochondria
mitochondria
Genome
Chloroplasts
genome
bundle sheath cells
chloroplasts
mesophyll
gas exchange
Organelle Size
Gases
Mitochondrial Size
Raphanus sativus

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

これを引用

Structural and biochemical dissection of photorespiration in hybrids differing in genome constitution between Diplotaxis tenuifolia (C3-C4) and radish (C3). / Ueno, Osamu; Bang, Sang Woo; Wada, Yoshiharu; Kondo, Ayumu; Ishihara, Kuni; Kaneko, Yukio; Matsuzawa, Yasuo.

:: Plant physiology, 巻 132, 番号 3, 01.07.2003, p. 1550-1559.

研究成果: ジャーナルへの寄稿記事

Ueno, Osamu ; Bang, Sang Woo ; Wada, Yoshiharu ; Kondo, Ayumu ; Ishihara, Kuni ; Kaneko, Yukio ; Matsuzawa, Yasuo. / Structural and biochemical dissection of photorespiration in hybrids differing in genome constitution between Diplotaxis tenuifolia (C3-C4) and radish (C3). :: Plant physiology. 2003 ; 巻 132, 番号 3. pp. 1550-1559.
@article{928f3db0deb049d19506bec9f1656ce9,
title = "Structural and biochemical dissection of photorespiration in hybrids differing in genome constitution between Diplotaxis tenuifolia (C3-C4) and radish (C3)",
abstract = "We compared the structural, biochemical, and physiological characteristics involved in photorespiration of intergeneric hybrids differing in genome constitution (DtDtR, DtDtRR, and DtRR) between the C3-C4 intermediate species Diplotaxis tenuifolia (DtDt) and the C3 species radish (Raphanus sativus; RR). The bundle sheath (BS) cells in D. tenuifolia included many centripetally located chloroplasts and mitochondria, but those of radish had only a few chloroplasts and mitochondria. In the hybrids, the numbers of chloroplasts and mitochondria, the ratio of centripetally located organdies to total organelles, and the mitochondrial size in the BS cells increased with an increase in the constitution ratio of the Dt:R genome. The P-protein of glycine decarboxylase (GDC) was confined to the BS mitochondria in D. tenuifolia, whereas in radish, it accumulated more densely in the mesophyll than in the BS mitochondria. In the hybrids, more intense accumulation of GDC in the BS relative to the mesophyll mitochondria occurred with an increase in the Dt:R ratio. These structural and biochemical features in the hybrids were reflected in the gas exchange characteristics of leaves, such as the CO2 compensation point. Our data indicate that the leaf structure, the intercellular pattern of GDC expression, and the gas exchange characteristics of C3-C4 intermediate photosynthesis are inherited in the hybrids depending on the constitution ratio of the parent genomes. Our findings also demonstrate that the apparent reduced photorespiration in C3-C4 intermediate plants is mainly due to the structural differentiation of mitochondria and chloroplasts in the BS cells combined with the BS-dominant expression of GDC.",
author = "Osamu Ueno and Bang, {Sang Woo} and Yoshiharu Wada and Ayumu Kondo and Kuni Ishihara and Yukio Kaneko and Yasuo Matsuzawa",
year = "2003",
month = "7",
day = "1",
doi = "10.1104/pp.103.021329",
language = "English",
volume = "132",
pages = "1550--1559",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "3",

}

TY - JOUR

T1 - Structural and biochemical dissection of photorespiration in hybrids differing in genome constitution between Diplotaxis tenuifolia (C3-C4) and radish (C3)

AU - Ueno, Osamu

AU - Bang, Sang Woo

AU - Wada, Yoshiharu

AU - Kondo, Ayumu

AU - Ishihara, Kuni

AU - Kaneko, Yukio

AU - Matsuzawa, Yasuo

PY - 2003/7/1

Y1 - 2003/7/1

N2 - We compared the structural, biochemical, and physiological characteristics involved in photorespiration of intergeneric hybrids differing in genome constitution (DtDtR, DtDtRR, and DtRR) between the C3-C4 intermediate species Diplotaxis tenuifolia (DtDt) and the C3 species radish (Raphanus sativus; RR). The bundle sheath (BS) cells in D. tenuifolia included many centripetally located chloroplasts and mitochondria, but those of radish had only a few chloroplasts and mitochondria. In the hybrids, the numbers of chloroplasts and mitochondria, the ratio of centripetally located organdies to total organelles, and the mitochondrial size in the BS cells increased with an increase in the constitution ratio of the Dt:R genome. The P-protein of glycine decarboxylase (GDC) was confined to the BS mitochondria in D. tenuifolia, whereas in radish, it accumulated more densely in the mesophyll than in the BS mitochondria. In the hybrids, more intense accumulation of GDC in the BS relative to the mesophyll mitochondria occurred with an increase in the Dt:R ratio. These structural and biochemical features in the hybrids were reflected in the gas exchange characteristics of leaves, such as the CO2 compensation point. Our data indicate that the leaf structure, the intercellular pattern of GDC expression, and the gas exchange characteristics of C3-C4 intermediate photosynthesis are inherited in the hybrids depending on the constitution ratio of the parent genomes. Our findings also demonstrate that the apparent reduced photorespiration in C3-C4 intermediate plants is mainly due to the structural differentiation of mitochondria and chloroplasts in the BS cells combined with the BS-dominant expression of GDC.

AB - We compared the structural, biochemical, and physiological characteristics involved in photorespiration of intergeneric hybrids differing in genome constitution (DtDtR, DtDtRR, and DtRR) between the C3-C4 intermediate species Diplotaxis tenuifolia (DtDt) and the C3 species radish (Raphanus sativus; RR). The bundle sheath (BS) cells in D. tenuifolia included many centripetally located chloroplasts and mitochondria, but those of radish had only a few chloroplasts and mitochondria. In the hybrids, the numbers of chloroplasts and mitochondria, the ratio of centripetally located organdies to total organelles, and the mitochondrial size in the BS cells increased with an increase in the constitution ratio of the Dt:R genome. The P-protein of glycine decarboxylase (GDC) was confined to the BS mitochondria in D. tenuifolia, whereas in radish, it accumulated more densely in the mesophyll than in the BS mitochondria. In the hybrids, more intense accumulation of GDC in the BS relative to the mesophyll mitochondria occurred with an increase in the Dt:R ratio. These structural and biochemical features in the hybrids were reflected in the gas exchange characteristics of leaves, such as the CO2 compensation point. Our data indicate that the leaf structure, the intercellular pattern of GDC expression, and the gas exchange characteristics of C3-C4 intermediate photosynthesis are inherited in the hybrids depending on the constitution ratio of the parent genomes. Our findings also demonstrate that the apparent reduced photorespiration in C3-C4 intermediate plants is mainly due to the structural differentiation of mitochondria and chloroplasts in the BS cells combined with the BS-dominant expression of GDC.

UR - http://www.scopus.com/inward/record.url?scp=0038038400&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038038400&partnerID=8YFLogxK

U2 - 10.1104/pp.103.021329

DO - 10.1104/pp.103.021329

M3 - Article

C2 - 12857835

AN - SCOPUS:0038038400

VL - 132

SP - 1550

EP - 1559

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 3

ER -