Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet /631/45 /631/57 /101 /82 /145 article

Sooho Choi, Pankaj Attri, Inhwan Lee, Jeongmin Oh, Ji Hye Yun, Ji Hoon Park, Eun Ha Choi, Weontae Lee

研究成果: ジャーナルへの寄稿学術誌査読

46 被引用数 (Scopus)

抄録

The variation in the biological function of proteins plays an important role in plasma medicine and sterilization. Several non-thermal plasma sources with different feeding gases are used worldwide for plasma treatment, including dielectric barrier discharge (DBD) and atmospheric-pressure plasma jet (APPJ) as the most commonly used sources. Therefore, in the present work, we used both DBD and APPJ plasma sources with N2 and air as feeding gases to evaluate the effects on the structural, thermodynamic, and activity changes of enzymes. In the current work, we used lysozyme as a model enzyme and verified the structural changes using circular dichroism (CD), fluorescence, and X-ray crystallography. In addition, we investigated the lysozyme thermodynamics using CD thermal analysis and changes in the B-factor from X-ray crystallography. The results showed that lysozyme activity decreased after the plasma treatment. From these analyses, we concluded that N2-feeding gas plasma disturbs the structure and activity of lysozyme more than Air feeding gas plasma in our experimental studies. This study provides novel fundamental information on the changes to enzymes upon plasma treatment, which has been absent from the literature until now.

本文言語英語
論文番号1027
ジャーナルScientific reports
7
1
DOI
出版ステータス出版済み - 12月 1 2017
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 一般

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