Structural basis for interaction between the conserved cell polarity proteins inscuteable and Leu-Gly-Asn repeat-enriched protein (LGN)

Satoru Yuzawa, Sachiko Kamakura, Yuko Iwakiri, Junya Hayase, Hideki Sumimoto

研究成果: Contribution to journalArticle査読

48 被引用数 (Scopus)

抄録

Interaction between the mammalian cell polarity proteins mInsc (mammalian homologue of Inscuteable) and Leu-Gly-Asn repeat-enriched protein (LGN), as well as that between their respective Drosophila homologues Inscuteable and Partner of Inscuteable (Pins), plays crucial roles in mitotic spindle orientation, a process contributing to asymmetric cell division. Here, we report a crystal structure of the LGN-binding domain (LBD) of human mInsc complexed with the N-terminal tetratricopeptide repeat (TPR) motifs of human LGN at 2.6-Å resolution. In the complex, mInsc-LBD adopts an elongated structure with three binding modules - an α-helix, an extended region, and a β-sheet connected with a loop - that runs antiparallel to LGN along the concave surface of the superhelix formed by the TPRs. Structural analysis and structure-based mutagenesis define residues that are critical for mInsc - LGN association, and reveal that the activator of G-protein signaling 3 (AGS3)-binding protein Frmpd1 [4.1/ezrin/radixin/moesin (FERM) and PSD-95/Dlg/ZO-1 (PDZ) domain-containing protein 1] and its relative Frmpd4 interact with LGN via a region homologous to a part of mInsc-LBD, whereas nuclear mitotic apparatus protein (NuMA) and the C terminus of LGN recognize the TPR domain in a manner different from that by mInsc. mInsc binds to LGN with the highest affinity (K D ≈ 2.4 nM) and effectively replaces the Frmpd proteins, NuMA, and the LGN C terminus, suggesting the priority of mInsc in binding to LGN. We also demonstrate, using mutant proteins, that mInsc-LGN interaction is vital for stabilization of LGN and for intracellular localization of mInsc.

本文言語英語
ページ(範囲)19210-19215
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
108
48
DOI
出版ステータス出版済み - 11 29 2011

All Science Journal Classification (ASJC) codes

  • General

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