Structural basis for the broad range substrate specificity of a novel mouse cytosolic sulfotransferase-mSULT1D1

Takamasa Teramoto, Yoichi Sakakibara, Ming Cheh Liu, Masahito Suiko, Makoto Kimura, Yoshimitsu Kakuta

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

The mouse cytosolic sulfotransferase, mSULT1D1, catalyzes the sulfonation of a wide range of phenolic molecules including p-nitrophenol (pNP), α-naphthol (αNT), serotonin, as well as dopamine and its metabolites. To gain insight into the structural basis for its broad range substrate specificity, we solved two distinct ternary crystal structures of mSULT1D1, complexed with 3′-phosphoadenosine-5′-phosphate (PAP) plus pNP or PAP plus αNT. The structures revealed that the mSULT1D1 contains an L-shaped accepter-binding site which comprises 20 amino acid residues and four conserved water molecules. The shape of the accepter-binding site can be adjusted by conformational changes of two residues, Ile148 and Glu247, upon binding with respective substrates.

元の言語英語
ページ(範囲)76-80
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
379
発行部数1
DOI
出版物ステータス出版済み - 1 30 2009

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Sulfotransferases
Naphthols
Substrate Specificity
Binding Sites
Sulfonation
Molecules
Substrates
Metabolites
Dopamine
Serotonin
Crystal structure
Phosphates
Amino Acids
Water
4-nitrophenol
adenosine 3'-phosphate-5'-phosphate

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

これを引用

Structural basis for the broad range substrate specificity of a novel mouse cytosolic sulfotransferase-mSULT1D1. / Teramoto, Takamasa; Sakakibara, Yoichi; Liu, Ming Cheh; Suiko, Masahito; Kimura, Makoto; Kakuta, Yoshimitsu.

:: Biochemical and Biophysical Research Communications, 巻 379, 番号 1, 30.01.2009, p. 76-80.

研究成果: ジャーナルへの寄稿記事

Teramoto, Takamasa ; Sakakibara, Yoichi ; Liu, Ming Cheh ; Suiko, Masahito ; Kimura, Makoto ; Kakuta, Yoshimitsu. / Structural basis for the broad range substrate specificity of a novel mouse cytosolic sulfotransferase-mSULT1D1. :: Biochemical and Biophysical Research Communications. 2009 ; 巻 379, 番号 1. pp. 76-80.
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AU - Kimura, Makoto

AU - Kakuta, Yoshimitsu

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