Structural characterization of α-terminal group of natural rubber. 1. Decomposition of branch-points by lipase and phosphatase treatments

Lucksanaporn Tarachiwin, Jitladda Sakdapipanich, Koichi Ute, Tatsuki Kitayama, Takashi Bamba, Ei Ichiro Fukusaki, Akio Kobayashi, Yasuyuki Tanaka

研究成果: ジャーナルへの寄稿記事

68 引用 (Scopus)

抄録

Deproteinized natural rubber latex (DPNR-latex) was treated with lipase and phosphatase in order to analyze the structure of the chain-end group (α-terminal). The enzymatic treatment decreased the content of long-chain fatty acid ester groups in DPNR from about 6 to 2 mol per rubber molecule. The molecular weight and intrinsic viscosity were reduced to about one-third after treatment with lipase and phosphatase. The Huggins' k′ constant of the enzyme-treated DPNR showed the formation of linear rubber molecules. The molecular weight distribution of DPNR changed apparently after treatment with lipase and phosphatase. 1H NMR spectrum of rubber obtained from DPNR-latex showed small signals due to monophosphate, diphosphate and phospholipids at the α-terminus. Treatment of DPNR-latex with lipase and phosphatase decreased the relative intensity of the 1H NMR signals corresponding to phospholipids, whereas no change was observed for the signals due to mono- and diphosphates. The residual mono- and diphosphate signals as well as some phospholipid signals after lipase and phosphatase treatments indicate that mono- and diphosphate groups are directly linked at the α-terminus with the modified structure, expected by aggregation or linking with phospholipid molecules.

元の言語英語
ページ(範囲)1851-1857
ページ数7
ジャーナルBiomacromolecules
6
発行部数4
DOI
出版物ステータス出版済み - 7 1 2005

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Phosphatases
Rubber
Lipases
Lipase
Phosphoric Monoester Hydrolases
Diphosphates
Phospholipids
Decomposition
Latex
Latexes
Molecules
Nuclear magnetic resonance
Molecular weight distribution
Fatty acids
Esters
Fatty Acids
Agglomeration
Enzymes
Molecular weight
Viscosity

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

これを引用

Structural characterization of α-terminal group of natural rubber. 1. Decomposition of branch-points by lipase and phosphatase treatments. / Tarachiwin, Lucksanaporn; Sakdapipanich, Jitladda; Ute, Koichi; Kitayama, Tatsuki; Bamba, Takashi; Fukusaki, Ei Ichiro; Kobayashi, Akio; Tanaka, Yasuyuki.

:: Biomacromolecules, 巻 6, 番号 4, 01.07.2005, p. 1851-1857.

研究成果: ジャーナルへの寄稿記事

Tarachiwin, L, Sakdapipanich, J, Ute, K, Kitayama, T, Bamba, T, Fukusaki, EI, Kobayashi, A & Tanaka, Y 2005, 'Structural characterization of α-terminal group of natural rubber. 1. Decomposition of branch-points by lipase and phosphatase treatments', Biomacromolecules, 巻. 6, 番号 4, pp. 1851-1857. https://doi.org/10.1021/bm058003x
Tarachiwin, Lucksanaporn ; Sakdapipanich, Jitladda ; Ute, Koichi ; Kitayama, Tatsuki ; Bamba, Takashi ; Fukusaki, Ei Ichiro ; Kobayashi, Akio ; Tanaka, Yasuyuki. / Structural characterization of α-terminal group of natural rubber. 1. Decomposition of branch-points by lipase and phosphatase treatments. :: Biomacromolecules. 2005 ; 巻 6, 番号 4. pp. 1851-1857.
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AU - Kitayama, Tatsuki

AU - Bamba, Takashi

AU - Fukusaki, Ei Ichiro

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