Structural characterization of nanoparticles from thermoresponsive poly(N-isopropylacrylamide)-DNA conjugate

Wei Yang Ooi, Masahiro Fujita, Pengju Pan, Hui Ying Tang, Kumar Sudesh, Kazuki Ito, Naoki Kanayama, Tohru Takarada, Mizuo Maeda

研究成果: ジャーナルへの寄稿記事

15 引用 (Scopus)

抄録

Poly(N-isopropylacrylamide) (PNIPAAm) grafted with single-stranded (ss) DNA conjugate (PNIPAAm-g-DNA) self-assembles above its lower critical solution temperature to form colloidal particles. When the ssDNA within the particle hybridizes with its complementary DNA, the particles aggregate above a certain threshold of salt concentration with drastically increased turbidity in solution. Detailed structural information of the particle was obtained mainly by small-angle X-ray scattering. The influence of copolymer composition on the morphology of particle and non-crosslinking aggregation was examined. The particle consists of hydrophobic PNIPAAm core surrounded by hydrophilic DNA strands. The increase in DNA fraction brought about a significant decrease in core size, whereas the shell thickness little changed and corresponded to the length of DNA. A structural model with a sticky potential was applied to the analysis of particle aggregate. This analysis provided that the particles aggregate while the coronal layers interpenetrate each other. The interaction between the particles was quantified in terms of the sticky potential and showed a trend to be influenced by the particle size rather than the graft density of DNA strands on the particle.

元の言語英語
ページ(範囲)315-320
ページ数6
ジャーナルJournal of Colloid And Interface Science
374
発行部数1
DOI
出版物ステータス出版済み - 5 15 2012

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DNA
Nanoparticles
Single-Stranded DNA
Turbidity
X ray scattering
Grafts
Agglomeration
Copolymers
Complementary DNA
Salts
Particle size
poly-N-isopropylacrylamide
Chemical analysis
Temperature

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry

これを引用

Structural characterization of nanoparticles from thermoresponsive poly(N-isopropylacrylamide)-DNA conjugate. / Ooi, Wei Yang; Fujita, Masahiro; Pan, Pengju; Tang, Hui Ying; Sudesh, Kumar; Ito, Kazuki; Kanayama, Naoki; Takarada, Tohru; Maeda, Mizuo.

:: Journal of Colloid And Interface Science, 巻 374, 番号 1, 15.05.2012, p. 315-320.

研究成果: ジャーナルへの寄稿記事

Ooi, Wei Yang ; Fujita, Masahiro ; Pan, Pengju ; Tang, Hui Ying ; Sudesh, Kumar ; Ito, Kazuki ; Kanayama, Naoki ; Takarada, Tohru ; Maeda, Mizuo. / Structural characterization of nanoparticles from thermoresponsive poly(N-isopropylacrylamide)-DNA conjugate. :: Journal of Colloid And Interface Science. 2012 ; 巻 374, 番号 1. pp. 315-320.
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abstract = "Poly(N-isopropylacrylamide) (PNIPAAm) grafted with single-stranded (ss) DNA conjugate (PNIPAAm-g-DNA) self-assembles above its lower critical solution temperature to form colloidal particles. When the ssDNA within the particle hybridizes with its complementary DNA, the particles aggregate above a certain threshold of salt concentration with drastically increased turbidity in solution. Detailed structural information of the particle was obtained mainly by small-angle X-ray scattering. The influence of copolymer composition on the morphology of particle and non-crosslinking aggregation was examined. The particle consists of hydrophobic PNIPAAm core surrounded by hydrophilic DNA strands. The increase in DNA fraction brought about a significant decrease in core size, whereas the shell thickness little changed and corresponded to the length of DNA. A structural model with a sticky potential was applied to the analysis of particle aggregate. This analysis provided that the particles aggregate while the coronal layers interpenetrate each other. The interaction between the particles was quantified in terms of the sticky potential and showed a trend to be influenced by the particle size rather than the graft density of DNA strands on the particle.",
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AU - Sudesh, Kumar

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