Structural Design of Oligopeptides for Intestinal Transport Model

Seong Min Hong, Mitsuru Tanaka, Riho Koyanagi, Weilin Shen, Toshiro Matsui

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

Glycyl-sarcosine (Gly-Sar) is a well-known model substrate for the intestinal uptake of dipeptides through peptide transporter 1 (PepT1). However, there are no other model peptides larger than tripeptides to evaluate their intestinal transport ability. In this study, we designed new oligopeptides based on the Gly-Sar structure in terms of protease resistance. Gly-Sar-Sar was found to be an appropriate transport model for tripeptides because it does not degrade during the transport across the rat intestinal membrane, while Gly-Gly-Sar was degraded to Gly-Sar during the 60 min transport. Caco-2 cell transport experiments revealed that the designed oligopeptides based on Gly-Sar-Sar showed a significantly (p < 0.05) lower transport ability by factors of 1/10-, 1/25-, and 1/40-fold for Gly-Sar-Sar, Gly-Sar-Sar-Sar, and Gly-Sar-Sar-Sar-Sar, respectively, compared to Gly-Sar (apparent permeability coefficient: 38.6 ± 11.4 cm/s). Cell experiments also showed that the designed tripeptide and Gly-Sar were transported across Caco-2 cell via PepT1, whereas the tetra- A nd pentapeptides were transported through the paracellular tight-junction pathway.

元の言語英語
ページ(範囲)2072-2079
ページ数8
ジャーナルJournal of Agricultural and Food Chemistry
64
発行部数10
DOI
出版物ステータス出版済み - 3 16 2016

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Sarcosine
oligopeptides
tripeptides
Oligopeptides
Structural design
peptide transporters
tight junctions
dipeptides
cells
permeability
proteinases
peptides
Caco-2 Cells
uptake mechanisms
rats
Dipeptides
Tight Junctions
Hydraulic conductivity
Permeability
Peptide Hydrolases

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

これを引用

Structural Design of Oligopeptides for Intestinal Transport Model. / Hong, Seong Min; Tanaka, Mitsuru; Koyanagi, Riho; Shen, Weilin; Matsui, Toshiro.

:: Journal of Agricultural and Food Chemistry, 巻 64, 番号 10, 16.03.2016, p. 2072-2079.

研究成果: ジャーナルへの寄稿記事

Hong, Seong Min ; Tanaka, Mitsuru ; Koyanagi, Riho ; Shen, Weilin ; Matsui, Toshiro. / Structural Design of Oligopeptides for Intestinal Transport Model. :: Journal of Agricultural and Food Chemistry. 2016 ; 巻 64, 番号 10. pp. 2072-2079.
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