Structural determinant for switching between the polymerase and exonuclease modes in the PCNA-replicative DNA polymerase complex

Hirokazu Nishida, Kouta Mayanagi, Shinichi Kiyonari, Yuichi Sato, Takuji Oyama, Yoshizumi Ishino, Kosuke Morikawa

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Proliferating cell nuclear antigen (PCNA) is responsible for the processivity of DNA polymerase. We determined the crystal structure of Pyrococcus furiosus DNA polymerase (PfuPol) complexed with the cognate monomeric PCNA, which allowed us to construct a convincing model of the polymerase-PCNA ring interaction, with unprecedented configurations of the two molecules. Electron microscopic analyses indicated that this complex structure exists in solution. Our structural study revealed that an interaction occurs between a stretched loop of PCNA and the PfuPol Thumb domain, in addition to the authentic PCNA-polymerase recognition site (PIP box). Comparisons of the present structure with the previously reported structures of polymerases complexed with DNA, suggested that the second interaction plays a crucial role in switching between the polymerase and exonuclease modes, by inducing a PCNA-polymerase complex configuration that favors synthesis over editing. This putative mechanism for fidelity control of replicative DNA polymerases is supported by experiments, in which mutations at the second interaction site caused enhancements in the exonuclease activity in the presence of PCNA.

元の言語英語
ページ(範囲)20693-20698
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
106
発行部数49
DOI
出版物ステータス出版済み - 12 8 2009

All Science Journal Classification (ASJC) codes

  • General

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