Structural organization of the bovine adrenodoxin gene

Yasuhiro Sagara, Hiromi Sawae, Akihumi Kimura, Yasuko Sagara-nakano, Ken-Ichirou Morohashi, Ken Ichi Miyoshi, Tadao Horiuchi

研究成果: ジャーナルへの寄稿記事

23 引用 (Scopus)

抄録

The gene structure of bovine adrenodoxin (Ad), a component of the steroid hydroxylating system in adrenal cortex mitochondria, was determined. This gene seems to be a single copy, but contains a pseudo exon in addition to the genuine gene region. The gene region for the active Ad is divided into 4 exons by 3 introns, and spans at least 27 kb. All the splice donor and acceptor sites follow the GT/AG rule. The transcription initiation site was determined by primer extension analysis, and putative TATA and GC boxes are present in the 5'-flanking region. A 12 base sequence, CCAGGGCCAGGG, is present 95 bases upstream from the transcription initiation site. A similar sequence was also found in the 5'-flanking regions of all genes for the steroid hydroxylating cytochromes P-450: P-450(SCC), P-450(11 β), P-450(17α), and P-450(C21), in the adrenal cortex. The consensus sequence for these five sequences is C GCAGGGCCA TGGG A. The pseudo exon, exon 1', was present about 0.5 kb upstream from exon 1. It encodes the prepeptide region of the minor type Ad mRNA, but has a stop codon (TAA) in the frame. A TATA-box-like sequence and a CAAT-box-like sequence are also present in the 5'-flanking region of exon 1'. The GC content of exon 1 was over 80%, whereas for each of the other four exons, including exon 1', it was about 40%. Our observations suggest that exon 1, which encodes the prepeptide region of the major type Ad mRNA, has intruded into the region between exon 1' and exon 2.

元の言語英語
ページ(範囲)77-83
ページ数7
ジャーナルJournal of Biochemistry
107
発行部数1
DOI
出版物ステータス出版済み - 1 1 1990

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Adrenodoxin
Exons
Genes
5' Flanking Region
TATA Box
RNA Splice Sites
Transcription Initiation Site
Adrenal Cortex
Steroids
Messenger RNA
Mitochondria
Terminator Codon
Consensus Sequence
Base Composition
Introns
Cytochrome P-450 Enzyme System

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

Sagara, Y., Sawae, H., Kimura, A., Sagara-nakano, Y., Morohashi, K-I., Miyoshi, K. I., & Horiuchi, T. (1990). Structural organization of the bovine adrenodoxin gene. Journal of Biochemistry, 107(1), 77-83. https://doi.org/10.1093/oxfordjournals.jbchem.a123015

Structural organization of the bovine adrenodoxin gene. / Sagara, Yasuhiro; Sawae, Hiromi; Kimura, Akihumi; Sagara-nakano, Yasuko; Morohashi, Ken-Ichirou; Miyoshi, Ken Ichi; Horiuchi, Tadao.

:: Journal of Biochemistry, 巻 107, 番号 1, 01.01.1990, p. 77-83.

研究成果: ジャーナルへの寄稿記事

Sagara, Y, Sawae, H, Kimura, A, Sagara-nakano, Y, Morohashi, K-I, Miyoshi, KI & Horiuchi, T 1990, 'Structural organization of the bovine adrenodoxin gene', Journal of Biochemistry, 巻. 107, 番号 1, pp. 77-83. https://doi.org/10.1093/oxfordjournals.jbchem.a123015
Sagara, Yasuhiro ; Sawae, Hiromi ; Kimura, Akihumi ; Sagara-nakano, Yasuko ; Morohashi, Ken-Ichirou ; Miyoshi, Ken Ichi ; Horiuchi, Tadao. / Structural organization of the bovine adrenodoxin gene. :: Journal of Biochemistry. 1990 ; 巻 107, 番号 1. pp. 77-83.
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abstract = "The gene structure of bovine adrenodoxin (Ad), a component of the steroid hydroxylating system in adrenal cortex mitochondria, was determined. This gene seems to be a single copy, but contains a pseudo exon in addition to the genuine gene region. The gene region for the active Ad is divided into 4 exons by 3 introns, and spans at least 27 kb. All the splice donor and acceptor sites follow the GT/AG rule. The transcription initiation site was determined by primer extension analysis, and putative TATA and GC boxes are present in the 5'-flanking region. A 12 base sequence, CCAGGGCCAGGG, is present 95 bases upstream from the transcription initiation site. A similar sequence was also found in the 5'-flanking regions of all genes for the steroid hydroxylating cytochromes P-450: P-450(SCC), P-450(11 β), P-450(17α), and P-450(C21), in the adrenal cortex. The consensus sequence for these five sequences is C GCAGGGCCA TGGG A. The pseudo exon, exon 1', was present about 0.5 kb upstream from exon 1. It encodes the prepeptide region of the minor type Ad mRNA, but has a stop codon (TAA) in the frame. A TATA-box-like sequence and a CAAT-box-like sequence are also present in the 5'-flanking region of exon 1'. The GC content of exon 1 was over 80{\%}, whereas for each of the other four exons, including exon 1', it was about 40{\%}. Our observations suggest that exon 1, which encodes the prepeptide region of the major type Ad mRNA, has intruded into the region between exon 1' and exon 2.",
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