Structural properties of silkworm small heat-shock proteins:SHSP19.9 and sHSP20.8

MD Tofazzal Hossain, Satoshi Teshiba, Yuichi Shigeoka, Tetsuro Fujisawa, Yoji Inoko, Daisuke Sakano, Kohji Yamamoto, Yutaka Banno, Yoichi Aso

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

sHSP20.8 and sHSP19.9 are silkworm small-heat shock proteins (sHSPs) comprising a number of polypeptides of molecular sizes of several tens of kilodaltons as subunits. The structural properties of sHSPs were investigated. sHSP19.9 was found to be aggregated by itself during incubation at 60 °C. Aggregation was suppressed in the presence of dithiothreitol and at high ionic strength. In contrast, sHSP20.8 was not aggregated. Aggregation of sHSP19.9 was partially suppressed by sHSP20.8 and in the presence of catalase as a target protein. Based on changes in small-angle X-ray scattering, it is possible that the molecular size of sHSP19.9 is larger than that of sHSP20.8, and that their molecular sizes increase with increasing temperature in a reversible, biphasic manner. sHSPs did not protect catalase from thermal inactivation, but protected it from precipitation by forming a soluble complex. sHSP20.8 and sHSP19.9 with dithiothreitol were stable against lyophilization, autoclaving at 120 °C, and boiling.

元の言語英語
ページ(範囲)1556-1563
ページ数8
ジャーナルBioscience, Biotechnology and Biochemistry
74
発行部数8
DOI
出版物ステータス出版済み - 9 1 2010

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Small Heat-Shock Proteins
Bombyx
Structural properties
Dithiothreitol
Catalase
Agglomeration
Freeze Drying
Ionic strength
X ray scattering
Osmolar Concentration
Boiling liquids
Hot Temperature
X-Rays
Peptides
Temperature
Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

Hossain, MD. T., Teshiba, S., Shigeoka, Y., Fujisawa, T., Inoko, Y., Sakano, D., ... Aso, Y. (2010). Structural properties of silkworm small heat-shock proteins:SHSP19.9 and sHSP20.8. Bioscience, Biotechnology and Biochemistry, 74(8), 1556-1563. https://doi.org/10.1271/bbb.100131

Structural properties of silkworm small heat-shock proteins:SHSP19.9 and sHSP20.8. / Hossain, MD Tofazzal; Teshiba, Satoshi; Shigeoka, Yuichi; Fujisawa, Tetsuro; Inoko, Yoji; Sakano, Daisuke; Yamamoto, Kohji; Banno, Yutaka; Aso, Yoichi.

:: Bioscience, Biotechnology and Biochemistry, 巻 74, 番号 8, 01.09.2010, p. 1556-1563.

研究成果: ジャーナルへの寄稿記事

Hossain, MDT, Teshiba, S, Shigeoka, Y, Fujisawa, T, Inoko, Y, Sakano, D, Yamamoto, K, Banno, Y & Aso, Y 2010, 'Structural properties of silkworm small heat-shock proteins:SHSP19.9 and sHSP20.8', Bioscience, Biotechnology and Biochemistry, 巻. 74, 番号 8, pp. 1556-1563. https://doi.org/10.1271/bbb.100131
Hossain MDT, Teshiba S, Shigeoka Y, Fujisawa T, Inoko Y, Sakano D その他. Structural properties of silkworm small heat-shock proteins:SHSP19.9 and sHSP20.8. Bioscience, Biotechnology and Biochemistry. 2010 9 1;74(8):1556-1563. https://doi.org/10.1271/bbb.100131
Hossain, MD Tofazzal ; Teshiba, Satoshi ; Shigeoka, Yuichi ; Fujisawa, Tetsuro ; Inoko, Yoji ; Sakano, Daisuke ; Yamamoto, Kohji ; Banno, Yutaka ; Aso, Yoichi. / Structural properties of silkworm small heat-shock proteins:SHSP19.9 and sHSP20.8. :: Bioscience, Biotechnology and Biochemistry. 2010 ; 巻 74, 番号 8. pp. 1556-1563.
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AU - Inoko, Yoji

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