Structure and expression analysis of the Cecropin-E gene from the silkworm, bombyx mori

Sun Mee Hong, Takahiro Kusakabe, Jae Man Lee, Tsuneyuki Tatsuke, Yutaka Kawaguchi, Min Wook Kang, Seok Woo Kang, Kyung A. Kim, Si Kab Nho

研究成果: Contribution to journalArticle査読

22 被引用数 (Scopus)

抄録

Cecropins belong to the antibacterial peptides family and are induced after injection of bacteria or their cell-wall components. By silkworm cDNA microarray analysis, a novel type of Cecropin family gene was identified as a cDNA up-regulated in early embryo, 1 day after oviposition. The cDNA isolated was 394 bp with 198 ORF translating 65 amino acids, encoding BmCecropin-E (BmCec-E). Using Southern hybridization and genome search analysis, the number of BmCec-E gene was estimated to be at least two per haploid, which consisted of two exons, as in other Cecropin family members. BmCec-E mRNA was expressed transiently 1 day after egg-laying (AEL, germ-band formation stage), and was specifically expressed in the degenerating intestine during the pre-pupal and pupal stages, unlike other Cecropin family genes. Immune challenge analysis showed that BmCec-E gene expression was more strongly induced by Escherichia coli (gram-negative) than by Micrococus luteus (gram-positive), and not by virus injection. By bacterial challenge, expression of BmCec-E mRNA was induced 12 h after injection, and was maintained for 24 h. Expression of BmCec-E after immune challenge was observed strongly in excretory organs, such as hindgut and malphigian, slightly in fat body, skin, and midgut.

本文言語英語
ページ(範囲)1992-1998
ページ数7
ジャーナルBioscience, Biotechnology and Biochemistry
72
8
DOI
出版ステータス出版済み - 2008

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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