Structure and expression of the alkA gene of Escherichia coli involved in adaptive response to alkylating agents

Y. Nakabeppu, T. Miyata, H. Kondo, S. Iwanaga, M. Sekiguchi

研究成果: ジャーナルへの寄稿記事

66 引用 (Scopus)

抄録

Nucleotide sequence of a DNA fragment containing the alkA gene and its control region has been determined using a chemical method. Only one open reading frame responsible for 3-methyladenine DNA glycosylase II was found. The hypothetical polypeptide deduced from the DNA sequence, with a molecular weight of 31,400, had an amino-terminal sequence and total amino acid composition identical to that of purified 4-methyladenine DNA glycosylase II. We constructed hybrid plasmids carrying an alkA'-lacZ' fusion, with the proper control region for alkA expression. A hybrid polypeptide with β-galactosidase activity was formed when lac mutant cells harboring such plasmids were incubated with low doses of N-methyl-N'-nitro-N'-nitrosoguanidine or methylmethane sulfonate. Other DNA-damaging agents, such as ethylmethane sulfonate, nalidixic acid, and ultraviolet light did not induce the enzyme activity. The induction was controlled by the ada and adc, but not by the recA and lexA genes.

元の言語英語
ページ(範囲)13730-13736
ページ数7
ジャーナルJournal of Biological Chemistry
259
発行部数22
出版物ステータス出版済み - 12 1 1984

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Alkylating Agents
Escherichia coli
Plasmids
Genes
Galactosidases
DNA Glycosylases
Ethyl Methanesulfonate
Methyl Methanesulfonate
Gene Expression
Methylnitronitrosoguanidine
Nalidixic Acid
Peptides
DNA sequences
DNA
Enzyme activity
Ultraviolet Rays
Open Reading Frames
Amino Acid Sequence
Fusion reactions
Nucleotides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Structure and expression of the alkA gene of Escherichia coli involved in adaptive response to alkylating agents. / Nakabeppu, Y.; Miyata, T.; Kondo, H.; Iwanaga, S.; Sekiguchi, M.

:: Journal of Biological Chemistry, 巻 259, 番号 22, 01.12.1984, p. 13730-13736.

研究成果: ジャーナルへの寄稿記事

Nakabeppu, Y. ; Miyata, T. ; Kondo, H. ; Iwanaga, S. ; Sekiguchi, M. / Structure and expression of the alkA gene of Escherichia coli involved in adaptive response to alkylating agents. :: Journal of Biological Chemistry. 1984 ; 巻 259, 番号 22. pp. 13730-13736.
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abstract = "Nucleotide sequence of a DNA fragment containing the alkA gene and its control region has been determined using a chemical method. Only one open reading frame responsible for 3-methyladenine DNA glycosylase II was found. The hypothetical polypeptide deduced from the DNA sequence, with a molecular weight of 31,400, had an amino-terminal sequence and total amino acid composition identical to that of purified 4-methyladenine DNA glycosylase II. We constructed hybrid plasmids carrying an alkA'-lacZ' fusion, with the proper control region for alkA expression. A hybrid polypeptide with β-galactosidase activity was formed when lac mutant cells harboring such plasmids were incubated with low doses of N-methyl-N'-nitro-N'-nitrosoguanidine or methylmethane sulfonate. Other DNA-damaging agents, such as ethylmethane sulfonate, nalidixic acid, and ultraviolet light did not induce the enzyme activity. The induction was controlled by the ada and adc, but not by the recA and lexA genes.",
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AU - Sekiguchi, M.

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N2 - Nucleotide sequence of a DNA fragment containing the alkA gene and its control region has been determined using a chemical method. Only one open reading frame responsible for 3-methyladenine DNA glycosylase II was found. The hypothetical polypeptide deduced from the DNA sequence, with a molecular weight of 31,400, had an amino-terminal sequence and total amino acid composition identical to that of purified 4-methyladenine DNA glycosylase II. We constructed hybrid plasmids carrying an alkA'-lacZ' fusion, with the proper control region for alkA expression. A hybrid polypeptide with β-galactosidase activity was formed when lac mutant cells harboring such plasmids were incubated with low doses of N-methyl-N'-nitro-N'-nitrosoguanidine or methylmethane sulfonate. Other DNA-damaging agents, such as ethylmethane sulfonate, nalidixic acid, and ultraviolet light did not induce the enzyme activity. The induction was controlled by the ada and adc, but not by the recA and lexA genes.

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