Structure and ligand recognition of the PB1 domain: A novel protein module binding to the PC motif

Hiroaki Terasawa, Yukiko Noda, Takashi Ito, Hideki Hatanaka, Saori Ichikawa, Kenji Ogura, Hideki Sumimoto, Fuyuhiko Inagaki

研究成果: ジャーナルへの寄稿記事

57 引用 (Scopus)

抄録

PB1 domains are novel protein modules capable of binding to target proteins that contain PC motifs. We report here the NMR structure and ligand-binding site of the PB1 domain of the cell polarity establishment protein, Bem1p. In addition, we identify the topology of the PC motif-containing region of Cdc24p by NMR, another cell polarity establishment protein that interacts with Bem1p. The PC motif-containing region is a structural domain offering a scaffold to the PC motif. The chemical shift perturbation experiment and the mutagenesis study show that the PC motif is a major structural element that binds to the PB1 domain. A structural database search reveals close similarity between the Bem1p PB1 domain and the c-Raf1 Ras-binding domain. However, these domains are functionally distinct from each other.

元の言語英語
ページ(範囲)3947-3956
ページ数10
ジャーナルEMBO Journal
20
発行部数15
DOI
出版物ステータス出版済み - 8 1 2001

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Protein Binding
Cell Polarity
Ligands
Proteins
Nuclear magnetic resonance
Mutagenesis
Chemical shift
Binding Sites
Scaffolds
Databases
Topology
Experiments

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

これを引用

Terasawa, H., Noda, Y., Ito, T., Hatanaka, H., Ichikawa, S., Ogura, K., ... Inagaki, F. (2001). Structure and ligand recognition of the PB1 domain: A novel protein module binding to the PC motif. EMBO Journal, 20(15), 3947-3956. https://doi.org/10.1093/emboj/20.15.3947

Structure and ligand recognition of the PB1 domain : A novel protein module binding to the PC motif. / Terasawa, Hiroaki; Noda, Yukiko; Ito, Takashi; Hatanaka, Hideki; Ichikawa, Saori; Ogura, Kenji; Sumimoto, Hideki; Inagaki, Fuyuhiko.

:: EMBO Journal, 巻 20, 番号 15, 01.08.2001, p. 3947-3956.

研究成果: ジャーナルへの寄稿記事

Terasawa, H, Noda, Y, Ito, T, Hatanaka, H, Ichikawa, S, Ogura, K, Sumimoto, H & Inagaki, F 2001, 'Structure and ligand recognition of the PB1 domain: A novel protein module binding to the PC motif', EMBO Journal, 巻. 20, 番号 15, pp. 3947-3956. https://doi.org/10.1093/emboj/20.15.3947
Terasawa, Hiroaki ; Noda, Yukiko ; Ito, Takashi ; Hatanaka, Hideki ; Ichikawa, Saori ; Ogura, Kenji ; Sumimoto, Hideki ; Inagaki, Fuyuhiko. / Structure and ligand recognition of the PB1 domain : A novel protein module binding to the PC motif. :: EMBO Journal. 2001 ; 巻 20, 番号 15. pp. 3947-3956.
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