Structure of a bovine gene for P-450c21 (steroid 21-hydroxylase) defines a novel cytochrome P-450 gene family

Bon Chu Chung, K. J. Matteson, W. L. Miller

研究成果: ジャーナルへの寄稿記事

86 引用 (Scopus)

抄録

P-450c21, a cytochrome P-450 enzyme [steroid 21-monooxygenase (steroid 21-hydroxylase), EC 1.14.99.10], mediates the 21-hydroxylation of glucocorticoid and mineralocorticoid hormones in the adrenal gland. The complete sequence of a bovine P-450c21 gene shows it is 3447 base pairs long and contains 10 exons. The intron/exon organization and encoded amino acid sequence indicate that P-450c21 represents a unique family of genes in the P-450 gene superfamily. Primer extension and S1 nuclease protection experiments identified several cap sites for initiation of transcription; the principal cap site produces mRNA with a 5' untranslated region only 11 bases long. S1 nuclease protection experiments confirm that P-450c21 is actively expressed in the adrenal and the testis, an organ not known to secrete 21-hydroxylated steroids.

元の言語英語
ページ(範囲)4243-4247
ページ数5
ジャーナルProceedings of the National Academy of Sciences of the United States of America
83
発行部数12
DOI
出版物ステータス出版済み - 1 1 1986

Fingerprint

Steroid 21-Hydroxylase
Cytochrome P-450 Enzyme System
Exons
Genes
Mineralocorticoids
5' Untranslated Regions
Transcription Initiation Site
Hydroxylation
Adrenal Glands
Base Pairing
Introns
Glucocorticoids
Testis
Amino Acid Sequence
Steroids
Hormones
Messenger RNA

All Science Journal Classification (ASJC) codes

  • General

これを引用

Structure of a bovine gene for P-450c21 (steroid 21-hydroxylase) defines a novel cytochrome P-450 gene family. / Chung, Bon Chu; Matteson, K. J.; Miller, W. L.

:: Proceedings of the National Academy of Sciences of the United States of America, 巻 83, 番号 12, 01.01.1986, p. 4243-4247.

研究成果: ジャーナルへの寄稿記事

@article{0ba19255f6b44a238dac194040441c29,
title = "Structure of a bovine gene for P-450c21 (steroid 21-hydroxylase) defines a novel cytochrome P-450 gene family",
abstract = "P-450c21, a cytochrome P-450 enzyme [steroid 21-monooxygenase (steroid 21-hydroxylase), EC 1.14.99.10], mediates the 21-hydroxylation of glucocorticoid and mineralocorticoid hormones in the adrenal gland. The complete sequence of a bovine P-450c21 gene shows it is 3447 base pairs long and contains 10 exons. The intron/exon organization and encoded amino acid sequence indicate that P-450c21 represents a unique family of genes in the P-450 gene superfamily. Primer extension and S1 nuclease protection experiments identified several cap sites for initiation of transcription; the principal cap site produces mRNA with a 5' untranslated region only 11 bases long. S1 nuclease protection experiments confirm that P-450c21 is actively expressed in the adrenal and the testis, an organ not known to secrete 21-hydroxylated steroids.",
author = "Chung, {Bon Chu} and Matteson, {K. J.} and Miller, {W. L.}",
year = "1986",
month = "1",
day = "1",
doi = "10.1073/pnas.83.12.4243",
language = "English",
volume = "83",
pages = "4243--4247",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "12",

}

TY - JOUR

T1 - Structure of a bovine gene for P-450c21 (steroid 21-hydroxylase) defines a novel cytochrome P-450 gene family

AU - Chung, Bon Chu

AU - Matteson, K. J.

AU - Miller, W. L.

PY - 1986/1/1

Y1 - 1986/1/1

N2 - P-450c21, a cytochrome P-450 enzyme [steroid 21-monooxygenase (steroid 21-hydroxylase), EC 1.14.99.10], mediates the 21-hydroxylation of glucocorticoid and mineralocorticoid hormones in the adrenal gland. The complete sequence of a bovine P-450c21 gene shows it is 3447 base pairs long and contains 10 exons. The intron/exon organization and encoded amino acid sequence indicate that P-450c21 represents a unique family of genes in the P-450 gene superfamily. Primer extension and S1 nuclease protection experiments identified several cap sites for initiation of transcription; the principal cap site produces mRNA with a 5' untranslated region only 11 bases long. S1 nuclease protection experiments confirm that P-450c21 is actively expressed in the adrenal and the testis, an organ not known to secrete 21-hydroxylated steroids.

AB - P-450c21, a cytochrome P-450 enzyme [steroid 21-monooxygenase (steroid 21-hydroxylase), EC 1.14.99.10], mediates the 21-hydroxylation of glucocorticoid and mineralocorticoid hormones in the adrenal gland. The complete sequence of a bovine P-450c21 gene shows it is 3447 base pairs long and contains 10 exons. The intron/exon organization and encoded amino acid sequence indicate that P-450c21 represents a unique family of genes in the P-450 gene superfamily. Primer extension and S1 nuclease protection experiments identified several cap sites for initiation of transcription; the principal cap site produces mRNA with a 5' untranslated region only 11 bases long. S1 nuclease protection experiments confirm that P-450c21 is actively expressed in the adrenal and the testis, an organ not known to secrete 21-hydroxylated steroids.

UR - http://www.scopus.com/inward/record.url?scp=0022455516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022455516&partnerID=8YFLogxK

U2 - 10.1073/pnas.83.12.4243

DO - 10.1073/pnas.83.12.4243

M3 - Article

C2 - 3487086

AN - SCOPUS:0022455516

VL - 83

SP - 4243

EP - 4247

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 12

ER -