Studies on molecular structure of biocompatible polymers by vibrational spectroscopy [16] protein adsorption onto different polymer films

Time-resolved IR spectra of protein adsorption process onto different polymer surface from buffer solution have been investigated by using an original in-situ attenuated total reflection infrared (ATR-IR) cell (Figure 1). Adsorbed protein is selectively observed, i.e. the signal from natural protein in solution is not detected by the method. Time-dependent spectral shape variation in the amide bands region (1700-1200cm^-1) was observed (Figure 2). The spectral variation and the kinetics of adsorption will be discussed in detail.