Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: I. Isolation and some properties of the glycoproteins

Takayuki Jikibara, Kaoru Takegawa, Shojiro Iwahara

研究成果: ジャーナルへの寄稿記事

19 引用 (Scopus)

抄録

An acidic heteropolysaccharide preparation derived from the mycelium of Fusarium sp. M7-1 was fractionated into two fractions, precipitable and nonprecipitable, by treatment with cetyltrimethylammonium bromide (Cetavlon). These two fractions were further purified to apparent homogeneity on ultracentrifugation by treatment with charcoal and gel filtration chromatographies. Two glycoproteins, precipitable GP I and nonprecipitable GP II, were obtained. The molecular weighs of GP I and GP II were estimated to be about 8.8×104 and 3.7×104 respectively, on gel filtration chromatography. Both GP I and GP II contained a high proportion of serine and threonine. Treatment of GP I and GP II with alkaline solution resulted in an increase in absorbance at 240 nm. Alkaline borohydride treatment markedly decreased the number of Beryl and threonyl residues and resulted in an increase in alanine and the formation of 2-aminobutyric acid. It also resulted in release of low and high molecular weight carbohydrate chains. From these results, we conclude that both GP I and GP II are glycoproteins with carbohydrate chains attached to the protein moiety through O-glycosidic linkages to the hydroxyl group of serine and/or threonine.

元の言語英語
ページ(範囲)225-229
ページ数5
ジャーナルJournal of biochemistry
111
発行部数2
DOI
出版物ステータス出版済み - 1 1 1992

Fingerprint

Uronic Acids
Fusarium
Threonine
Chromatography
Serine
Gel Chromatography
Glycoproteins
Gels
Carbohydrates
Borohydrides
Charcoal
Mycelium
Ultracentrifugation
Alanine
Hydroxyl Radical
Molecular Weight
Molecular weight
Proteins
cetrimonium
beryl

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1 : I. Isolation and some properties of the glycoproteins. / Jikibara, Takayuki; Takegawa, Kaoru; Iwahara, Shojiro.

:: Journal of biochemistry, 巻 111, 番号 2, 01.01.1992, p. 225-229.

研究成果: ジャーナルへの寄稿記事

@article{9a4c1693b8cf486fb842768c89d21218,
title = "Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: I. Isolation and some properties of the glycoproteins",
abstract = "An acidic heteropolysaccharide preparation derived from the mycelium of Fusarium sp. M7-1 was fractionated into two fractions, precipitable and nonprecipitable, by treatment with cetyltrimethylammonium bromide (Cetavlon). These two fractions were further purified to apparent homogeneity on ultracentrifugation by treatment with charcoal and gel filtration chromatographies. Two glycoproteins, precipitable GP I and nonprecipitable GP II, were obtained. The molecular weighs of GP I and GP II were estimated to be about 8.8×104 and 3.7×104 respectively, on gel filtration chromatography. Both GP I and GP II contained a high proportion of serine and threonine. Treatment of GP I and GP II with alkaline solution resulted in an increase in absorbance at 240 nm. Alkaline borohydride treatment markedly decreased the number of Beryl and threonyl residues and resulted in an increase in alanine and the formation of 2-aminobutyric acid. It also resulted in release of low and high molecular weight carbohydrate chains. From these results, we conclude that both GP I and GP II are glycoproteins with carbohydrate chains attached to the protein moiety through O-glycosidic linkages to the hydroxyl group of serine and/or threonine.",
author = "Takayuki Jikibara and Kaoru Takegawa and Shojiro Iwahara",
year = "1992",
month = "1",
day = "1",
doi = "10.1093/oxfordjournals.jbchem.a123741",
language = "English",
volume = "111",
pages = "225--229",
journal = "Journal of Biochemistry",
issn = "0021-924X",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1

T2 - I. Isolation and some properties of the glycoproteins

AU - Jikibara, Takayuki

AU - Takegawa, Kaoru

AU - Iwahara, Shojiro

PY - 1992/1/1

Y1 - 1992/1/1

N2 - An acidic heteropolysaccharide preparation derived from the mycelium of Fusarium sp. M7-1 was fractionated into two fractions, precipitable and nonprecipitable, by treatment with cetyltrimethylammonium bromide (Cetavlon). These two fractions were further purified to apparent homogeneity on ultracentrifugation by treatment with charcoal and gel filtration chromatographies. Two glycoproteins, precipitable GP I and nonprecipitable GP II, were obtained. The molecular weighs of GP I and GP II were estimated to be about 8.8×104 and 3.7×104 respectively, on gel filtration chromatography. Both GP I and GP II contained a high proportion of serine and threonine. Treatment of GP I and GP II with alkaline solution resulted in an increase in absorbance at 240 nm. Alkaline borohydride treatment markedly decreased the number of Beryl and threonyl residues and resulted in an increase in alanine and the formation of 2-aminobutyric acid. It also resulted in release of low and high molecular weight carbohydrate chains. From these results, we conclude that both GP I and GP II are glycoproteins with carbohydrate chains attached to the protein moiety through O-glycosidic linkages to the hydroxyl group of serine and/or threonine.

AB - An acidic heteropolysaccharide preparation derived from the mycelium of Fusarium sp. M7-1 was fractionated into two fractions, precipitable and nonprecipitable, by treatment with cetyltrimethylammonium bromide (Cetavlon). These two fractions were further purified to apparent homogeneity on ultracentrifugation by treatment with charcoal and gel filtration chromatographies. Two glycoproteins, precipitable GP I and nonprecipitable GP II, were obtained. The molecular weighs of GP I and GP II were estimated to be about 8.8×104 and 3.7×104 respectively, on gel filtration chromatography. Both GP I and GP II contained a high proportion of serine and threonine. Treatment of GP I and GP II with alkaline solution resulted in an increase in absorbance at 240 nm. Alkaline borohydride treatment markedly decreased the number of Beryl and threonyl residues and resulted in an increase in alanine and the formation of 2-aminobutyric acid. It also resulted in release of low and high molecular weight carbohydrate chains. From these results, we conclude that both GP I and GP II are glycoproteins with carbohydrate chains attached to the protein moiety through O-glycosidic linkages to the hydroxyl group of serine and/or threonine.

UR - http://www.scopus.com/inward/record.url?scp=0026500323&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026500323&partnerID=8YFLogxK

U2 - 10.1093/oxfordjournals.jbchem.a123741

DO - 10.1093/oxfordjournals.jbchem.a123741

M3 - Article

C2 - 1569046

AN - SCOPUS:0026500323

VL - 111

SP - 225

EP - 229

JO - Journal of Biochemistry

JF - Journal of Biochemistry

SN - 0021-924X

IS - 2

ER -