Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: III. The primary structures of the acidic polysaccharides of the glycoproteins

Takayuki Jikibara, Kaoru Takegawa, Shojiro Iwahara

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Two O-glycosidically linked acidic polysaccharides, AP I and AP II, were released, respectively, from glycoproteins GP I and GP II of Fusarium sp. M7-1 by alkaline borohydride treatment and purified by gel filtration chromatography. They were found to be apparently homogeneous on gel filtration chromatography and analytical ultracentrifugation. Their molecular weights were estimated to be 8.2×104 and 3.1×104 respectively. The various oligosaccharide fragments obtained from AP I and AP II by acetolysis and partial acid hydrolysis were purified by gel filtration chromatography and HPLC. Their primary structures were resolved mainly by NMR spectrometry in combination with methylation mass spectrometry. Analyses of the acetolysis and partial acid hydrolysis products and the 1H-NMR spectrum of AP I and AP II showed that they are analogues. Thus, we propose that the main parts of the acidic polysaccharides have the following structures.*X, unidentified oligosaccharide chains. The numbers on the left and the numbers in parentheses outside the brackets indicate the approximate number of side chains of AP I and AP II, respectively, the saccharide sequences of which are not specified.

元の言語英語
ページ(範囲)236-243
ページ数8
ジャーナルJournal of biochemistry
111
発行部数2
DOI
出版物ステータス出版済み - 1 1 1992
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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