TY - GEN
T1 - Study on enhanced oil recovery using microorganism generating foam in presence of nanobubbles
AU - Ito, Miu
AU - Sugai, Yuichi
N1 - Funding Information:
This work was supported by JSPS KAKENHI Grant Number 19K05352. We are grateful to Dr. K. Sasaki and Dr. R. Nguele for helpful discussions and comments on the manuscript.
Publisher Copyright:
© 2021, Society of Petroleum Engineers.
PY - 2021
Y1 - 2021
N2 - Both high cost and environmental load of surfactant are issues to be solved in foam EOR. Moreover, it is difficult to control the injection of surfactant and gas so that the foam is generated in only high permeable zones selectively in oil reservoir. The authors have found a foam generating microorganism and hit upon an idea of the microbial foam EOR which makes the microorganism do generating foam in oil reservoir. The mechanism of the microbial foam generation and culture condition suitable for the foam generation were studied in this study. A species of Pseudomonas aeruginosa was used as a foam producer in this study. It was cultured in the medium consisting of glucose and eight kinds of minerals at 30 °C and atmospheric pressure under anaerobic conditions. Because P. aeruginosa generally grows better under aerobic conditions, the microorganism was supplied with oxygen nanobubbles as the oxygen source. The carbon dioxide nanobubbles were also used as a comparison target in this study. The state of foam generation in the culture solution was observed during the cultivation. The surface tension, surfactant concentration, protein concentration, polysaccharides concentration and bacterial population of the culture solution were measured respectively. The foam was started to be generated by the microorganism after 2 days of cultivation and its volume became maximum after 3 days of cultivation. The foam generation was found in the culture solution which contained both oxygen nanobubbles and carbon dioxide nanobubbles whereas little foam was found in non-nanobubbles culture solution. The foam generation found in the culture solution containing carbon dioxide nanobubbles was more than that in the culture solution containing oxygen nanobubbles. Both gas and protein concentration increased along with the formation of the foam whereas surfactant and polysaccharides were not increased, therefore, the foam was assumed to be generated with gas and protein which were generated by P. aeruginosa. It was found that the carbon dioxide nanobubbles were positively charged in the culture medium whereas they were negatively charged in tap water through the measurement of zeta potential of nanobubbles, therefore, the carbon dioxide nanobubbles attracted cations in the culture medium and became positively charged. Positively charged carbon dioxide nanobubbles transported cations to the microbial cells of P. aeruginosa. Among cations in the culture medium, ferrous ions are essential for the protein generation of P. aeruginosa, therefore, the positively charged carbon dioxide nanobubbles attracted ferrous ions and transport them to the microbial cells, resulting the growth and metabolism of P. aeruginosa were activated. Those results suggest that the microbial foam EOR can be materialized by supplying the microorganism with carbon dioxide nanobubbles or ferrous ions.
AB - Both high cost and environmental load of surfactant are issues to be solved in foam EOR. Moreover, it is difficult to control the injection of surfactant and gas so that the foam is generated in only high permeable zones selectively in oil reservoir. The authors have found a foam generating microorganism and hit upon an idea of the microbial foam EOR which makes the microorganism do generating foam in oil reservoir. The mechanism of the microbial foam generation and culture condition suitable for the foam generation were studied in this study. A species of Pseudomonas aeruginosa was used as a foam producer in this study. It was cultured in the medium consisting of glucose and eight kinds of minerals at 30 °C and atmospheric pressure under anaerobic conditions. Because P. aeruginosa generally grows better under aerobic conditions, the microorganism was supplied with oxygen nanobubbles as the oxygen source. The carbon dioxide nanobubbles were also used as a comparison target in this study. The state of foam generation in the culture solution was observed during the cultivation. The surface tension, surfactant concentration, protein concentration, polysaccharides concentration and bacterial population of the culture solution were measured respectively. The foam was started to be generated by the microorganism after 2 days of cultivation and its volume became maximum after 3 days of cultivation. The foam generation was found in the culture solution which contained both oxygen nanobubbles and carbon dioxide nanobubbles whereas little foam was found in non-nanobubbles culture solution. The foam generation found in the culture solution containing carbon dioxide nanobubbles was more than that in the culture solution containing oxygen nanobubbles. Both gas and protein concentration increased along with the formation of the foam whereas surfactant and polysaccharides were not increased, therefore, the foam was assumed to be generated with gas and protein which were generated by P. aeruginosa. It was found that the carbon dioxide nanobubbles were positively charged in the culture medium whereas they were negatively charged in tap water through the measurement of zeta potential of nanobubbles, therefore, the carbon dioxide nanobubbles attracted cations in the culture medium and became positively charged. Positively charged carbon dioxide nanobubbles transported cations to the microbial cells of P. aeruginosa. Among cations in the culture medium, ferrous ions are essential for the protein generation of P. aeruginosa, therefore, the positively charged carbon dioxide nanobubbles attracted ferrous ions and transport them to the microbial cells, resulting the growth and metabolism of P. aeruginosa were activated. Those results suggest that the microbial foam EOR can be materialized by supplying the microorganism with carbon dioxide nanobubbles or ferrous ions.
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U2 - 10.2118/205671-MS
DO - 10.2118/205671-MS
M3 - Conference contribution
AN - SCOPUS:85118463055
T3 - Society of Petroleum Engineers - SPE/IATMI Asia Pacific Oil and Gas Conference and Exhibition 2021, APOG 2021
BT - Society of Petroleum Engineers - SPE/IATMI Asia Pacific Oil and Gas Conference and Exhibition 2021, APOG 2021
PB - Society of Petroleum Engineers
T2 - SPE/IATMI Asia Pacific Oil and Gas Conference and Exhibition 2021, APOG 2021
Y2 - 12 October 2021 through 14 October 2021
ER -