D -Glutamate is metabolized in the heart mitochondria

Makoto Ariyoshi, Masumi Katane, Kenji Hamase, Yurika Miyoshi, Maiko Nakane, Atsushi Hoshino, Yoshifumi Okawa, Yuichiro Mita, Satoshi Kaimoto, Motoki Uchihashi, Kuniyoshi Fukai, Kazunori Ono, Syuhei Tateishi, Daichi Hato, Ryoetsu Yamanaka, Sakiko Honda, Yohei Fushimura, Eri Iwai-Kanai, Naotada Ishihara, Masashi Mita & 2 others Hiroshi Homma, Satoaki Matoba

研究成果: ジャーナルへの寄稿記事

18 引用 (Scopus)

抄録

D -Amino acids are enantiomers of L-amino acids and have recently been recognized as biomarkers and bioactive substances in mammals, including humans. In the present study, we investigated functions of the novel mammalian mitochondrial protein 9030617O03Rik and showed decreased expression under conditions of heart failure. Genomic sequence analyses showed partial homology with a bacterial aspartate/glutamate/hydantoin racemase. Subsequent determinations of all free amino acid concentrations in 9030617O03Rik-deficient mice showed high accumulations of D-glutamate in heart tissues. This is the first time that a significant amount of D-glutamate was detected in mammalian tissue. Further analysis of D-glutamate metabolism indicated that 9030617O03Rik is a D-glutamate cyclase that converts D-glutamate to 5-oxo-D-proline. Hence, this protein is the first identified enzyme responsible for mammalian D-glutamate metabolism, as confirmed in cloning analyses. These findings suggest that D-glutamate and 5-oxo-D-proline have bioactivities in mammals through the metabolism by D-glutamate cyclase.

元の言語英語
記事番号43911
ジャーナルScientific reports
7
DOI
出版物ステータス出版済み - 3 7 2017

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Heart Mitochondria
Glutamic Acid
glutamate racemase
Amino Acids
Proline
Mammals
Mitochondrial Proteins
Aspartic Acid
Sequence Analysis
Organism Cloning
Heart Failure
Biomarkers
Enzymes
Proteins

All Science Journal Classification (ASJC) codes

  • General

これを引用

Ariyoshi, M., Katane, M., Hamase, K., Miyoshi, Y., Nakane, M., Hoshino, A., ... Matoba, S. (2017). D -Glutamate is metabolized in the heart mitochondria Scientific reports, 7, [43911]. https://doi.org/10.1038/srep43911

D -Glutamate is metabolized in the heart mitochondria . / Ariyoshi, Makoto; Katane, Masumi; Hamase, Kenji; Miyoshi, Yurika; Nakane, Maiko; Hoshino, Atsushi; Okawa, Yoshifumi; Mita, Yuichiro; Kaimoto, Satoshi; Uchihashi, Motoki; Fukai, Kuniyoshi; Ono, Kazunori; Tateishi, Syuhei; Hato, Daichi; Yamanaka, Ryoetsu; Honda, Sakiko; Fushimura, Yohei; Iwai-Kanai, Eri; Ishihara, Naotada; Mita, Masashi; Homma, Hiroshi; Matoba, Satoaki.

:: Scientific reports, 巻 7, 43911, 07.03.2017.

研究成果: ジャーナルへの寄稿記事

Ariyoshi, M, Katane, M, Hamase, K, Miyoshi, Y, Nakane, M, Hoshino, A, Okawa, Y, Mita, Y, Kaimoto, S, Uchihashi, M, Fukai, K, Ono, K, Tateishi, S, Hato, D, Yamanaka, R, Honda, S, Fushimura, Y, Iwai-Kanai, E, Ishihara, N, Mita, M, Homma, H & Matoba, S 2017, 'D -Glutamate is metabolized in the heart mitochondria ', Scientific reports, 巻. 7, 43911. https://doi.org/10.1038/srep43911
Ariyoshi M, Katane M, Hamase K, Miyoshi Y, Nakane M, Hoshino A その他. D -Glutamate is metabolized in the heart mitochondria Scientific reports. 2017 3 7;7. 43911. https://doi.org/10.1038/srep43911
Ariyoshi, Makoto ; Katane, Masumi ; Hamase, Kenji ; Miyoshi, Yurika ; Nakane, Maiko ; Hoshino, Atsushi ; Okawa, Yoshifumi ; Mita, Yuichiro ; Kaimoto, Satoshi ; Uchihashi, Motoki ; Fukai, Kuniyoshi ; Ono, Kazunori ; Tateishi, Syuhei ; Hato, Daichi ; Yamanaka, Ryoetsu ; Honda, Sakiko ; Fushimura, Yohei ; Iwai-Kanai, Eri ; Ishihara, Naotada ; Mita, Masashi ; Homma, Hiroshi ; Matoba, Satoaki. / D -Glutamate is metabolized in the heart mitochondria :: Scientific reports. 2017 ; 巻 7.
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AU - Okawa, Yoshifumi

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AU - Fushimura, Yohei

AU - Iwai-Kanai, Eri

AU - Ishihara, Naotada

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AU - Homma, Hiroshi

AU - Matoba, Satoaki

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