TY - JOUR
T1 - Subnuclear gene positioning through lamina association affects copper tolerance
AU - Sakamoto, Yuki
AU - Sato, Mayuko
AU - Sato, Yoshikatsu
AU - Harada, Akihito
AU - Suzuki, Takamasa
AU - Goto, Chieko
AU - Tamura, Kentaro
AU - Toyooka, Kiminori
AU - Kimura, Hiroshi
AU - Ohkawa, Yasuyuki
AU - Hara-Nishimura, Ikuko
AU - Takagi, Shingo
AU - Matsunaga, Sachihiro
N1 - Funding Information:
We thank Dr. Nakagawa (Shimane Univ., Japan) for kindly providing pGWB vectors, Dr. Kakimoto (Osaka Univ., Japan) for kindly providing the pMM1 binary vector, and Ms. Wakazaki (RIKEN, Japan) for support in the TEM analysis. This research was supported by MEXT/JSPS KAKENHI (15H05955, 15H05962 and 19H03259), Research Grant in Natural Sciences of Mitsubishi Foundation and Novartis Foundation for the Promotion of Science to S.M., by MEXT/JSPS KAKENHI (18K14743) and the Sasakawa Scientific Research Grant from the Japan Science Society to Y. Sakamoto, and by MEXT/JSPS KAKENHI (15H05776) to I.H.-N. H.K. and Y.O. were supported by JST CREST (JPMJCR16G1) and MEXT/JSPS KAKENHI (18H05527). This work was also supported by Advanced Bioimaging Support in MEXT/JSPS KAKENHI (16H06280). We thank Jennifer Smith from Edanz Group (www.edanzediting.com/ac) for editing a draft of this manuscript.
Publisher Copyright:
© 2020, The Author(s).
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/12
Y1 - 2020/12
N2 - The nuclear lamina plays an important role in the regulation of chromatin organization and gene positioning in animals. CROWDED NUCLEI (CRWN) is a strong candidate for the plant nuclear lamina protein in Arabidopsis thaliana but its biological function was largely unknown. Here, we show that CRWNs localize at the nuclear lamina and build the meshwork structure. Fluorescence in situ hybridization and RNA-seq analyses revealed that CRWNs regulate chromatin distribution and gene expression. More than 2000 differentially expressed genes were identified in the crwn1crwn4 double mutant. Copper-associated (CA) genes that form a gene cluster on chromosome 5 were among the downregulated genes in the double mutant exhibiting low tolerance to excess copper. Our analyses showed this low tolerance to copper was associated with the suppression of CA gene expression and that CRWN1 interacts with the CA gene locus, enabling the locus to localize at the nuclear lamina under excess copper conditions.
AB - The nuclear lamina plays an important role in the regulation of chromatin organization and gene positioning in animals. CROWDED NUCLEI (CRWN) is a strong candidate for the plant nuclear lamina protein in Arabidopsis thaliana but its biological function was largely unknown. Here, we show that CRWNs localize at the nuclear lamina and build the meshwork structure. Fluorescence in situ hybridization and RNA-seq analyses revealed that CRWNs regulate chromatin distribution and gene expression. More than 2000 differentially expressed genes were identified in the crwn1crwn4 double mutant. Copper-associated (CA) genes that form a gene cluster on chromosome 5 were among the downregulated genes in the double mutant exhibiting low tolerance to excess copper. Our analyses showed this low tolerance to copper was associated with the suppression of CA gene expression and that CRWN1 interacts with the CA gene locus, enabling the locus to localize at the nuclear lamina under excess copper conditions.
UR - http://www.scopus.com/inward/record.url?scp=85096288756&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85096288756&partnerID=8YFLogxK
U2 - 10.1038/s41467-020-19621-z
DO - 10.1038/s41467-020-19621-z
M3 - Article
C2 - 33219233
AN - SCOPUS:85096288756
VL - 11
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 5914
ER -