Substrate Concentration Dependence of Apparent Maximum Reaction Rate and Michaelis Constant Observed in Immobilized Enzyme Reactions

研究成果: Contribution to journalArticle査読

12 被引用数 (Scopus)

抄録

Expressions for apparent kinetic parameters Vappm ane Kappm observed in immobilized enzyme reactions with diffusional and electrostatic effects are proposed on the basis of the equation of a tangent to the curve of a Sb/υ versus Sb plot. The validity of these expressions is shown by comparing the expression for Kappm with two approximate expressions proposed by other workers for the reaction catalyzed by an enzyme immobilized on the external surface of a nonporous support. Calculation showed that the effect of substrate concentration on Vappm and Kappm is basically different for systems where an enzyme is immobilized on the external and pore surfaces respectively of supports.

本文言語英語
ページ(範囲)123-129
ページ数7
ジャーナルkagaku kogaku ronbunshu
16
1
DOI
出版ステータス出版済み - 1990

All Science Journal Classification (ASJC) codes

  • 化学 (全般)
  • 化学工学(全般)

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