Subtypes of metabotropic glutamate receptors in the nucleus of the solitary tract of rats

Kiyoshi Matsumura, Takuya Tsuchihashi, Shuntaro Kagiyama, Isao Abe, Masatoshi Fujishima

研究成果: ジャーナルへの寄稿記事

26 引用 (Scopus)

抄録

We have determined the role of subgroups of metabotropic glutamate receptors (mGluRs) in the nucleus of the solitary tract (NTS) of normotensive Wistar rats. Unilateral microinjection of (S)-3,5-dihydroxyphenylglycine (3,5-DHPG), an agonist of group I mGluRs, into the NTS significantly decreased mean arterial pressure (MAP), heart rate (HR), and renal sympathetic nerve activity (RSNA) (-19.4 ± 2.6 mmHg, -16.4 ± 5.1 beats/min, and -30.6 ± 5.7% by 1 nmol). Microinjection of (R,S)-1-aminoindan-1,5- dicarboxylic acid (AIDA; 1 nmol), a putative antagonist of group I mGluRs, into the NTS caused transient decreases in MAP and RSNA, followed by sustained increases in MAP (+8.3 ± 2.4 mmHg) and RSNA (+27.7 ± 10.8%). Pretreatment with AIDA failed to prevent the cardiovascular and RSNA responses to microinjection of 3,5-DHPG. Unilateral microinjection of (S)-4- carboxy-3-hydroxyphenylglycine (4C3HPG), an agonist of group II mGluRs, into the NTS also significantly decreased MAP, HR, and RSNA, whose responses were not inhibited by pre-microinjection of (2S)-α-ethylglutamic acid (EGLU; 2 nmol), a putative antagonist of group II mGluRs. On the other hand, unilateral microinjection of L(+)-2-amino-4-phosphonobutyric acid (L-AP4), an agonist of group III mGluRs, into the NTS caused dose-related decreases in MAP (-8.3 ± 1.5 mmHg by 0.1 nmol and -45.1 ± 3.4 mmHg by 0.3 nmol), HR, and RSNA (-21.3 ± 3.9% by 0.1 nmol and -77.2 ± 6.5% by 0.3 nmol), whose responses were suppressed by pre-microinjection of (R,S)-α-cyclopropyl-4- phosphonophenylglycine (CPPG; 0.3 nmol), an antagonist of group III mGluRs. These results suggest that all subgroups of mGluRs participate in cardiovascular and sympathetic regulations in the NTS of rats, and that endogenous group I mGluRs in the NTS may contribute to tonic cardiovascular and sympathetic regulations.

元の言語英語
ページ(範囲)461-468
ページ数8
ジャーナルBrain Research
842
発行部数2
DOI
出版物ステータス出版済み - 9 25 1999

Fingerprint

Metabotropic Glutamate Receptors
Solitary Nucleus
Microinjections
Arterial Pressure
Kidney
Heart Rate
Wistar Rats
Acids

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

これを引用

Matsumura, K., Tsuchihashi, T., Kagiyama, S., Abe, I., & Fujishima, M. (1999). Subtypes of metabotropic glutamate receptors in the nucleus of the solitary tract of rats. Brain Research, 842(2), 461-468. https://doi.org/10.1016/S0006-8993(99)01889-2

Subtypes of metabotropic glutamate receptors in the nucleus of the solitary tract of rats. / Matsumura, Kiyoshi; Tsuchihashi, Takuya; Kagiyama, Shuntaro; Abe, Isao; Fujishima, Masatoshi.

:: Brain Research, 巻 842, 番号 2, 25.09.1999, p. 461-468.

研究成果: ジャーナルへの寄稿記事

Matsumura, K, Tsuchihashi, T, Kagiyama, S, Abe, I & Fujishima, M 1999, 'Subtypes of metabotropic glutamate receptors in the nucleus of the solitary tract of rats', Brain Research, 巻. 842, 番号 2, pp. 461-468. https://doi.org/10.1016/S0006-8993(99)01889-2
Matsumura, Kiyoshi ; Tsuchihashi, Takuya ; Kagiyama, Shuntaro ; Abe, Isao ; Fujishima, Masatoshi. / Subtypes of metabotropic glutamate receptors in the nucleus of the solitary tract of rats. :: Brain Research. 1999 ; 巻 842, 番号 2. pp. 461-468.
@article{a7b42572b6d640c8b40096f2344db388,
title = "Subtypes of metabotropic glutamate receptors in the nucleus of the solitary tract of rats",
abstract = "We have determined the role of subgroups of metabotropic glutamate receptors (mGluRs) in the nucleus of the solitary tract (NTS) of normotensive Wistar rats. Unilateral microinjection of (S)-3,5-dihydroxyphenylglycine (3,5-DHPG), an agonist of group I mGluRs, into the NTS significantly decreased mean arterial pressure (MAP), heart rate (HR), and renal sympathetic nerve activity (RSNA) (-19.4 ± 2.6 mmHg, -16.4 ± 5.1 beats/min, and -30.6 ± 5.7{\%} by 1 nmol). Microinjection of (R,S)-1-aminoindan-1,5- dicarboxylic acid (AIDA; 1 nmol), a putative antagonist of group I mGluRs, into the NTS caused transient decreases in MAP and RSNA, followed by sustained increases in MAP (+8.3 ± 2.4 mmHg) and RSNA (+27.7 ± 10.8{\%}). Pretreatment with AIDA failed to prevent the cardiovascular and RSNA responses to microinjection of 3,5-DHPG. Unilateral microinjection of (S)-4- carboxy-3-hydroxyphenylglycine (4C3HPG), an agonist of group II mGluRs, into the NTS also significantly decreased MAP, HR, and RSNA, whose responses were not inhibited by pre-microinjection of (2S)-α-ethylglutamic acid (EGLU; 2 nmol), a putative antagonist of group II mGluRs. On the other hand, unilateral microinjection of L(+)-2-amino-4-phosphonobutyric acid (L-AP4), an agonist of group III mGluRs, into the NTS caused dose-related decreases in MAP (-8.3 ± 1.5 mmHg by 0.1 nmol and -45.1 ± 3.4 mmHg by 0.3 nmol), HR, and RSNA (-21.3 ± 3.9{\%} by 0.1 nmol and -77.2 ± 6.5{\%} by 0.3 nmol), whose responses were suppressed by pre-microinjection of (R,S)-α-cyclopropyl-4- phosphonophenylglycine (CPPG; 0.3 nmol), an antagonist of group III mGluRs. These results suggest that all subgroups of mGluRs participate in cardiovascular and sympathetic regulations in the NTS of rats, and that endogenous group I mGluRs in the NTS may contribute to tonic cardiovascular and sympathetic regulations.",
author = "Kiyoshi Matsumura and Takuya Tsuchihashi and Shuntaro Kagiyama and Isao Abe and Masatoshi Fujishima",
year = "1999",
month = "9",
day = "25",
doi = "10.1016/S0006-8993(99)01889-2",
language = "English",
volume = "842",
pages = "461--468",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Subtypes of metabotropic glutamate receptors in the nucleus of the solitary tract of rats

AU - Matsumura, Kiyoshi

AU - Tsuchihashi, Takuya

AU - Kagiyama, Shuntaro

AU - Abe, Isao

AU - Fujishima, Masatoshi

PY - 1999/9/25

Y1 - 1999/9/25

N2 - We have determined the role of subgroups of metabotropic glutamate receptors (mGluRs) in the nucleus of the solitary tract (NTS) of normotensive Wistar rats. Unilateral microinjection of (S)-3,5-dihydroxyphenylglycine (3,5-DHPG), an agonist of group I mGluRs, into the NTS significantly decreased mean arterial pressure (MAP), heart rate (HR), and renal sympathetic nerve activity (RSNA) (-19.4 ± 2.6 mmHg, -16.4 ± 5.1 beats/min, and -30.6 ± 5.7% by 1 nmol). Microinjection of (R,S)-1-aminoindan-1,5- dicarboxylic acid (AIDA; 1 nmol), a putative antagonist of group I mGluRs, into the NTS caused transient decreases in MAP and RSNA, followed by sustained increases in MAP (+8.3 ± 2.4 mmHg) and RSNA (+27.7 ± 10.8%). Pretreatment with AIDA failed to prevent the cardiovascular and RSNA responses to microinjection of 3,5-DHPG. Unilateral microinjection of (S)-4- carboxy-3-hydroxyphenylglycine (4C3HPG), an agonist of group II mGluRs, into the NTS also significantly decreased MAP, HR, and RSNA, whose responses were not inhibited by pre-microinjection of (2S)-α-ethylglutamic acid (EGLU; 2 nmol), a putative antagonist of group II mGluRs. On the other hand, unilateral microinjection of L(+)-2-amino-4-phosphonobutyric acid (L-AP4), an agonist of group III mGluRs, into the NTS caused dose-related decreases in MAP (-8.3 ± 1.5 mmHg by 0.1 nmol and -45.1 ± 3.4 mmHg by 0.3 nmol), HR, and RSNA (-21.3 ± 3.9% by 0.1 nmol and -77.2 ± 6.5% by 0.3 nmol), whose responses were suppressed by pre-microinjection of (R,S)-α-cyclopropyl-4- phosphonophenylglycine (CPPG; 0.3 nmol), an antagonist of group III mGluRs. These results suggest that all subgroups of mGluRs participate in cardiovascular and sympathetic regulations in the NTS of rats, and that endogenous group I mGluRs in the NTS may contribute to tonic cardiovascular and sympathetic regulations.

AB - We have determined the role of subgroups of metabotropic glutamate receptors (mGluRs) in the nucleus of the solitary tract (NTS) of normotensive Wistar rats. Unilateral microinjection of (S)-3,5-dihydroxyphenylglycine (3,5-DHPG), an agonist of group I mGluRs, into the NTS significantly decreased mean arterial pressure (MAP), heart rate (HR), and renal sympathetic nerve activity (RSNA) (-19.4 ± 2.6 mmHg, -16.4 ± 5.1 beats/min, and -30.6 ± 5.7% by 1 nmol). Microinjection of (R,S)-1-aminoindan-1,5- dicarboxylic acid (AIDA; 1 nmol), a putative antagonist of group I mGluRs, into the NTS caused transient decreases in MAP and RSNA, followed by sustained increases in MAP (+8.3 ± 2.4 mmHg) and RSNA (+27.7 ± 10.8%). Pretreatment with AIDA failed to prevent the cardiovascular and RSNA responses to microinjection of 3,5-DHPG. Unilateral microinjection of (S)-4- carboxy-3-hydroxyphenylglycine (4C3HPG), an agonist of group II mGluRs, into the NTS also significantly decreased MAP, HR, and RSNA, whose responses were not inhibited by pre-microinjection of (2S)-α-ethylglutamic acid (EGLU; 2 nmol), a putative antagonist of group II mGluRs. On the other hand, unilateral microinjection of L(+)-2-amino-4-phosphonobutyric acid (L-AP4), an agonist of group III mGluRs, into the NTS caused dose-related decreases in MAP (-8.3 ± 1.5 mmHg by 0.1 nmol and -45.1 ± 3.4 mmHg by 0.3 nmol), HR, and RSNA (-21.3 ± 3.9% by 0.1 nmol and -77.2 ± 6.5% by 0.3 nmol), whose responses were suppressed by pre-microinjection of (R,S)-α-cyclopropyl-4- phosphonophenylglycine (CPPG; 0.3 nmol), an antagonist of group III mGluRs. These results suggest that all subgroups of mGluRs participate in cardiovascular and sympathetic regulations in the NTS of rats, and that endogenous group I mGluRs in the NTS may contribute to tonic cardiovascular and sympathetic regulations.

UR - http://www.scopus.com/inward/record.url?scp=0032826542&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032826542&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(99)01889-2

DO - 10.1016/S0006-8993(99)01889-2

M3 - Article

C2 - 10526143

AN - SCOPUS:0032826542

VL - 842

SP - 461

EP - 468

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -