Subtyping of Listeria monocytogenes Based on Nucleotide Polymorphism in the clpC, inlA, hlyA, and plcA Genes and Rapid Identification of L. monocytogenes Genetically Similar to Clinical Isolates

Ken-ichi Honjoh, Kumiko Fujihara, Takahiro Haraguchi, Yukari Ono, Hiroshi Kobayashi, Takahisa Miyamoto, Hiroshi Hiwaki, Hideaki Kamikado, Sung Sik Jang, Sangryeol Ryu

研究成果: Contribution to journalArticle査読

10 被引用数 (Scopus)

抄録

To develop a new method for identification of Listeria monocytogenes genetically similar to clinical isolates, single-nucleotide polymorphism (SNP) typing and multi-locus sequence typing (MLST) of 126 isolates of L. monocytogenes from clinical and environmental samples were performed based on sequence analysis of parts of four genes (hlyA, clpC, inlA, and plcA). Based on the sequences of the isolates in this study, SNP typing showed that hlyA, clpC, inlA, and plcA genes were categorized into 9, 14, 17, and 21 types, respectively. MLST showed that the isolates were grouped into 35 types including 12 types of clinical isolates. Out of those, four MLST types were found in food or environmental and clinical isolates. In particular, all clinical isolates with serotype 1/2a were grouped into the same hlyA SNP A5 type. A method using real-time PCR combined with Cycling Probe Technology was developed for rapid identification of SNP type of L. monocytogenes genetically similar to the clinical isolates. By using this method, the 1/2a clinical isolates showing MLST-2 were successfully identified with a specific primer set and a cycling probe designed on the basis of sequence of hlyA. Furthermore, clinical isolates of serotype 4b showing MLST-4 or -35 were successfully identified by a method using cycling probes based on sequences of clpC and inlA.

本文言語英語
ページ(範囲)557-564
ページ数8
ジャーナルFood Science and Technology Research
14
6
DOI
出版ステータス出版済み - 2008

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 食品科学
  • 化学工学(全般)
  • マーケティング
  • 産業および生産工学

フィンガープリント

「Subtyping of <i>Listeria monocytogenes</i> Based on Nucleotide Polymorphism in the <i>clpC, inlA, hlyA</i>, and <i>plcA</i> Genes and Rapid Identification of <i>L. monocytogenes</i> Genetically Similar to Clinical Isolates」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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