Successful and optimized in vivo gene transfer to rabbit carotid artery mediated by electronic pulse

takuya matsumoto, K. Komori, T. Shoji, S. Kuma, M. Kume, T. Yamaoka, E. Mori, Tadashi Furuyama, Yoshikazu Yonemitsu, K. Sugimachi

研究成果: ジャーナルへの寄稿記事

37 引用 (Scopus)

抄録

Several gene transfer methods, including viral or nonviral vehicles have been developed, however, efficacy, safety or handling continue to present problems. We developed a nonviral and plasmid-based method for arterial gene transfer by in vivo electronic pulse, using a newly designed T-shaped electrode. Using rabbit carotid arteries, we first optimized gene transfer efficiency, and firefly luciferase gene transfer via electronic pulse under 20 voltage (the pulse length: Pon time 20 ms, the pulse interval: Poff time 80 ms, number of pulse: 10 times) showed the highest gene expression. Exogenous gene expression was detectable for at least up to 14 days. Electroporation-mediated gene transfer of E. coli lacZ with nuclear localizing signal revealed successful gene transfer to luminal endothelial cells and to medial cells. Histological damage was recognized as the voltage was increased but neointima formation 4 weeks after gene transfer was not induced. In vivo electroporation-mediated arterial gene transfer is readily facilitated, is safe and may prove to be an alternative form of gene transfer to the vasculature.

元の言語英語
ページ(範囲)1174-1179
ページ数6
ジャーナルGene Therapy
8
発行部数15
DOI
出版物ステータス出版済み - 9 3 2001

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Carotid Arteries
Pulse
Rabbits
Genes
Electroporation
Firefly Luciferases
Gene Expression
Neointima
Electrodes
Plasmids
Endothelial Cells
Escherichia coli
Safety

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Genetics

これを引用

matsumoto, T., Komori, K., Shoji, T., Kuma, S., Kume, M., Yamaoka, T., ... Sugimachi, K. (2001). Successful and optimized in vivo gene transfer to rabbit carotid artery mediated by electronic pulse. Gene Therapy, 8(15), 1174-1179. https://doi.org/10.1038/sj.gt.3301502

Successful and optimized in vivo gene transfer to rabbit carotid artery mediated by electronic pulse. / matsumoto, takuya; Komori, K.; Shoji, T.; Kuma, S.; Kume, M.; Yamaoka, T.; Mori, E.; Furuyama, Tadashi; Yonemitsu, Yoshikazu; Sugimachi, K.

:: Gene Therapy, 巻 8, 番号 15, 03.09.2001, p. 1174-1179.

研究成果: ジャーナルへの寄稿記事

matsumoto, T, Komori, K, Shoji, T, Kuma, S, Kume, M, Yamaoka, T, Mori, E, Furuyama, T, Yonemitsu, Y & Sugimachi, K 2001, 'Successful and optimized in vivo gene transfer to rabbit carotid artery mediated by electronic pulse', Gene Therapy, 巻. 8, 番号 15, pp. 1174-1179. https://doi.org/10.1038/sj.gt.3301502
matsumoto, takuya ; Komori, K. ; Shoji, T. ; Kuma, S. ; Kume, M. ; Yamaoka, T. ; Mori, E. ; Furuyama, Tadashi ; Yonemitsu, Yoshikazu ; Sugimachi, K. / Successful and optimized in vivo gene transfer to rabbit carotid artery mediated by electronic pulse. :: Gene Therapy. 2001 ; 巻 8, 番号 15. pp. 1174-1179.
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