Sugar-carrying Polystyrenes Facilitate Harvesting of APCs from MLRs

Possible Application of Sugar-carrying Polystyrenes to Immunotherapy

Akira Imaizumi, Hideya Ohnishi, Akio Yamasaki, Makoto Kawamoto, Takashi Morisaki, Mitsuaki Goto, Masamichi Iwama, Toshihiro Akaike, Kenichiro Hasumi

研究成果: ジャーナルへの寄稿記事

抄録

Antigen-presenting cells (APCs) play a pivotal role in cancer immunotherapy. APCs in conventionally used flasks are harvested by enzymatic digestion or cell scraping for application to cancer immunotherapy. However, these methods may impair functional molecules expressed on the APC surface and reduce their effects in cancer immunotherapy. Recently, we found that APCs could be harvested by shaking at 4°C in flasks coated with poly[N-p-vinylbenzyl-O-2-acetoamide-2-deoxy-β-D-glucopyranosyl-(1→4)-2-acetoamide-2-deoxy-β-D-gluconamide] (PVGlcNAc) or a copolymer consisting of sulfonylurea (SU) linked to poly[N-p-vinyl-benzyl-4-O-β-D-galactopyranosyl-D-gluconamide] [P(VLA-co-SU)]. In the present study, we compared the functions of cytotoxic T-lymphocytes (CTLs) induced by APCs generated in PVGlcNAc- or P(VLA-co-SU)-coated flasks and conventional flasks. APCs from PVGlcNAc- or P(VLA-co-SU)-coated flasks showed higher expression of cluster of differentiation (CD)80/86, CD11c, and major histocompatibility complex class II alloantigen I-A(d), and higher cytotoxicity than APCs from conventional flasks. These results suggest that the use of PVGlcNAc- or P(VLA-co-SU)-coated flasks is optimal for harvesting APCs. The generated APCs also have a higher antigen-presenting ability compared to those generated in conventional flasks. Our results may contribute to the development of effective cancer immunotherapies.

元の言語英語
ページ(範囲)673-676
ページ数4
ジャーナルAnticancer research
36
発行部数2
出版物ステータス出版済み - 2 1 2016
外部発表Yes

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Polystyrenes
Antigen-Presenting Cells
Immunotherapy
Neoplasms
Isoantigens
Cytotoxic T-Lymphocytes
Major Histocompatibility Complex
Digestion
Antigens
poly(N-p-vinylbenzyl-O-2-acetoamide-2-deoxyglucopyranosyl-(1-4)-2-acetoamide-2-deoxygluconamide)

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

これを引用

Sugar-carrying Polystyrenes Facilitate Harvesting of APCs from MLRs : Possible Application of Sugar-carrying Polystyrenes to Immunotherapy. / Imaizumi, Akira; Ohnishi, Hideya; Yamasaki, Akio; Kawamoto, Makoto; Morisaki, Takashi; Goto, Mitsuaki; Iwama, Masamichi; Akaike, Toshihiro; Hasumi, Kenichiro.

:: Anticancer research, 巻 36, 番号 2, 01.02.2016, p. 673-676.

研究成果: ジャーナルへの寄稿記事

Imaizumi, A, Ohnishi, H, Yamasaki, A, Kawamoto, M, Morisaki, T, Goto, M, Iwama, M, Akaike, T & Hasumi, K 2016, 'Sugar-carrying Polystyrenes Facilitate Harvesting of APCs from MLRs: Possible Application of Sugar-carrying Polystyrenes to Immunotherapy', Anticancer research, 巻. 36, 番号 2, pp. 673-676.
Imaizumi, Akira ; Ohnishi, Hideya ; Yamasaki, Akio ; Kawamoto, Makoto ; Morisaki, Takashi ; Goto, Mitsuaki ; Iwama, Masamichi ; Akaike, Toshihiro ; Hasumi, Kenichiro. / Sugar-carrying Polystyrenes Facilitate Harvesting of APCs from MLRs : Possible Application of Sugar-carrying Polystyrenes to Immunotherapy. :: Anticancer research. 2016 ; 巻 36, 番号 2. pp. 673-676.
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title = "Sugar-carrying Polystyrenes Facilitate Harvesting of APCs from MLRs: Possible Application of Sugar-carrying Polystyrenes to Immunotherapy",
abstract = "Antigen-presenting cells (APCs) play a pivotal role in cancer immunotherapy. APCs in conventionally used flasks are harvested by enzymatic digestion or cell scraping for application to cancer immunotherapy. However, these methods may impair functional molecules expressed on the APC surface and reduce their effects in cancer immunotherapy. Recently, we found that APCs could be harvested by shaking at 4°C in flasks coated with poly[N-p-vinylbenzyl-O-2-acetoamide-2-deoxy-β-D-glucopyranosyl-(1→4)-2-acetoamide-2-deoxy-β-D-gluconamide] (PVGlcNAc) or a copolymer consisting of sulfonylurea (SU) linked to poly[N-p-vinyl-benzyl-4-O-β-D-galactopyranosyl-D-gluconamide] [P(VLA-co-SU)]. In the present study, we compared the functions of cytotoxic T-lymphocytes (CTLs) induced by APCs generated in PVGlcNAc- or P(VLA-co-SU)-coated flasks and conventional flasks. APCs from PVGlcNAc- or P(VLA-co-SU)-coated flasks showed higher expression of cluster of differentiation (CD)80/86, CD11c, and major histocompatibility complex class II alloantigen I-A(d), and higher cytotoxicity than APCs from conventional flasks. These results suggest that the use of PVGlcNAc- or P(VLA-co-SU)-coated flasks is optimal for harvesting APCs. The generated APCs also have a higher antigen-presenting ability compared to those generated in conventional flasks. Our results may contribute to the development of effective cancer immunotherapies.",
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T2 - Possible Application of Sugar-carrying Polystyrenes to Immunotherapy

AU - Imaizumi, Akira

AU - Ohnishi, Hideya

AU - Yamasaki, Akio

AU - Kawamoto, Makoto

AU - Morisaki, Takashi

AU - Goto, Mitsuaki

AU - Iwama, Masamichi

AU - Akaike, Toshihiro

AU - Hasumi, Kenichiro

PY - 2016/2/1

Y1 - 2016/2/1

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