Sulfolobus tokodaii ST0053 produces a novel thermostable, NAD-dependent medium-chain alcohol dehydrogenase

Hisaaki Yanai, Katsumi Doi, Toshihisa Ohshima

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

An alcohol dehydrogenase (ADH) gene, ST0053, from Sulfolobus tokodaii was expressed In Escherichia coli. The purified recombinant enzyme was an NAD-dependent medium-chain ADH with high thermostability and tolerance of a wide range of pHs. This is the first step in creating an experimental functionality library of 10 genes annotated as ADHs in the S. tokodaii genome.

元の言語英語
ページ(範囲)1758-1763
ページ数6
ジャーナルApplied and environmental microbiology
75
発行部数6
DOI
出版物ステータス出版済み - 3 1 2009

Fingerprint

Sulfolobus
Alcohol Dehydrogenase
alcohol dehydrogenase
NAD
alcohol
gene
thermal stability
Gene Library
genes
genome
tolerance
Genome
enzyme
Escherichia coli
Enzymes
enzymes
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

これを引用

Sulfolobus tokodaii ST0053 produces a novel thermostable, NAD-dependent medium-chain alcohol dehydrogenase. / Yanai, Hisaaki; Doi, Katsumi; Ohshima, Toshihisa.

:: Applied and environmental microbiology, 巻 75, 番号 6, 01.03.2009, p. 1758-1763.

研究成果: ジャーナルへの寄稿記事

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