抄録
An alcohol dehydrogenase (ADH) gene, ST0053, from Sulfolobus tokodaii was expressed In Escherichia coli. The purified recombinant enzyme was an NAD-dependent medium-chain ADH with high thermostability and tolerance of a wide range of pHs. This is the first step in creating an experimental functionality library of 10 genes annotated as ADHs in the S. tokodaii genome.
元の言語 | 英語 |
---|---|
ページ(範囲) | 1758-1763 |
ページ数 | 6 |
ジャーナル | Applied and environmental microbiology |
巻 | 75 |
発行部数 | 6 |
DOI | |
出版物ステータス | 出版済み - 3 1 2009 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Food Science
- Applied Microbiology and Biotechnology
- Ecology
これを引用
Sulfolobus tokodaii ST0053 produces a novel thermostable, NAD-dependent medium-chain alcohol dehydrogenase. / Yanai, Hisaaki; Doi, Katsumi; Ohshima, Toshihisa.
:: Applied and environmental microbiology, 巻 75, 番号 6, 01.03.2009, p. 1758-1763.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Sulfolobus tokodaii ST0053 produces a novel thermostable, NAD-dependent medium-chain alcohol dehydrogenase
AU - Yanai, Hisaaki
AU - Doi, Katsumi
AU - Ohshima, Toshihisa
PY - 2009/3/1
Y1 - 2009/3/1
N2 - An alcohol dehydrogenase (ADH) gene, ST0053, from Sulfolobus tokodaii was expressed In Escherichia coli. The purified recombinant enzyme was an NAD-dependent medium-chain ADH with high thermostability and tolerance of a wide range of pHs. This is the first step in creating an experimental functionality library of 10 genes annotated as ADHs in the S. tokodaii genome.
AB - An alcohol dehydrogenase (ADH) gene, ST0053, from Sulfolobus tokodaii was expressed In Escherichia coli. The purified recombinant enzyme was an NAD-dependent medium-chain ADH with high thermostability and tolerance of a wide range of pHs. This is the first step in creating an experimental functionality library of 10 genes annotated as ADHs in the S. tokodaii genome.
UR - http://www.scopus.com/inward/record.url?scp=62149103359&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=62149103359&partnerID=8YFLogxK
U2 - 10.1128/AEM.01392-08
DO - 10.1128/AEM.01392-08
M3 - Article
C2 - 19139244
AN - SCOPUS:62149103359
VL - 75
SP - 1758
EP - 1763
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 6
ER -