13C-NMR relaxation study on mobility of the DNA-binding arm of HU

Yoshimitsu Kakuta, Hironobu Hojo, Saburo Aimoto, Isao Tanaka, Kunio Hikichi

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

The mobility of the DNA-binding arm of HU protein was studied by 13C-NMR spectroscopy. The correlation times τc of Phe47Cα in the body and Gly60Cα in the arm of HU were determined for HU and HU-DNA complex. The value of τc of Phe47Cα is 2-4 times larger than that of Gly60Cα irrespective of the presence or absence of DNA. These results show that Gly60Cα undergoes more rapid motion than Phe47Cα. The increase in correlation time on addition of DNA is greater for Gly60Cα than for Phe47Cα. This suggests that the addition of DNA influences more significantly the motion of Gly60Cα than that of Phe47Cα. These results are in accord with the X-ray result, in which the top part of the arm is not visible. Gly60Cα in the arm is thus more mobile than Phe47Cα in the body, and the mobility of Gly60Cα is reduced by the DNA binding.

元の言語英語
ページ(範囲)1153-1155
ページ数3
ジャーナルJournal of biochemistry
116
発行部数5
DOI
出版物ステータス出版済み - 1 1 1994

Fingerprint

Nuclear magnetic resonance
DNA
Nuclear magnetic resonance spectroscopy
Carbon-13 Magnetic Resonance Spectroscopy
Magnetic Resonance Spectroscopy
X-Rays
X rays
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

13C-NMR relaxation study on mobility of the DNA-binding arm of HU. / Kakuta, Yoshimitsu; Hojo, Hironobu; Aimoto, Saburo; Tanaka, Isao; Hikichi, Kunio.

:: Journal of biochemistry, 巻 116, 番号 5, 01.01.1994, p. 1153-1155.

研究成果: ジャーナルへの寄稿記事

Kakuta, Yoshimitsu ; Hojo, Hironobu ; Aimoto, Saburo ; Tanaka, Isao ; Hikichi, Kunio. / 13C-NMR relaxation study on mobility of the DNA-binding arm of HU. :: Journal of biochemistry. 1994 ; 巻 116, 番号 5. pp. 1153-1155.
@article{66b5298ac42445c599fa21670dedc510,
title = "13C-NMR relaxation study on mobility of the DNA-binding arm of HU",
abstract = "The mobility of the DNA-binding arm of HU protein was studied by 13C-NMR spectroscopy. The correlation times τc of Phe47Cα in the body and Gly60Cα in the arm of HU were determined for HU and HU-DNA complex. The value of τc of Phe47Cα is 2-4 times larger than that of Gly60Cα irrespective of the presence or absence of DNA. These results show that Gly60Cα undergoes more rapid motion than Phe47Cα. The increase in correlation time on addition of DNA is greater for Gly60Cα than for Phe47Cα. This suggests that the addition of DNA influences more significantly the motion of Gly60Cα than that of Phe47Cα. These results are in accord with the X-ray result, in which the top part of the arm is not visible. Gly60Cα in the arm is thus more mobile than Phe47Cα in the body, and the mobility of Gly60Cα is reduced by the DNA binding.",
author = "Yoshimitsu Kakuta and Hironobu Hojo and Saburo Aimoto and Isao Tanaka and Kunio Hikichi",
year = "1994",
month = "1",
day = "1",
doi = "10.1093/oxfordjournals.jbchem.a124642",
language = "English",
volume = "116",
pages = "1153--1155",
journal = "Journal of Biochemistry",
issn = "0021-924X",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - 13C-NMR relaxation study on mobility of the DNA-binding arm of HU

AU - Kakuta, Yoshimitsu

AU - Hojo, Hironobu

AU - Aimoto, Saburo

AU - Tanaka, Isao

AU - Hikichi, Kunio

PY - 1994/1/1

Y1 - 1994/1/1

N2 - The mobility of the DNA-binding arm of HU protein was studied by 13C-NMR spectroscopy. The correlation times τc of Phe47Cα in the body and Gly60Cα in the arm of HU were determined for HU and HU-DNA complex. The value of τc of Phe47Cα is 2-4 times larger than that of Gly60Cα irrespective of the presence or absence of DNA. These results show that Gly60Cα undergoes more rapid motion than Phe47Cα. The increase in correlation time on addition of DNA is greater for Gly60Cα than for Phe47Cα. This suggests that the addition of DNA influences more significantly the motion of Gly60Cα than that of Phe47Cα. These results are in accord with the X-ray result, in which the top part of the arm is not visible. Gly60Cα in the arm is thus more mobile than Phe47Cα in the body, and the mobility of Gly60Cα is reduced by the DNA binding.

AB - The mobility of the DNA-binding arm of HU protein was studied by 13C-NMR spectroscopy. The correlation times τc of Phe47Cα in the body and Gly60Cα in the arm of HU were determined for HU and HU-DNA complex. The value of τc of Phe47Cα is 2-4 times larger than that of Gly60Cα irrespective of the presence or absence of DNA. These results show that Gly60Cα undergoes more rapid motion than Phe47Cα. The increase in correlation time on addition of DNA is greater for Gly60Cα than for Phe47Cα. This suggests that the addition of DNA influences more significantly the motion of Gly60Cα than that of Phe47Cα. These results are in accord with the X-ray result, in which the top part of the arm is not visible. Gly60Cα in the arm is thus more mobile than Phe47Cα in the body, and the mobility of Gly60Cα is reduced by the DNA binding.

UR - http://www.scopus.com/inward/record.url?scp=0028171443&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028171443&partnerID=8YFLogxK

U2 - 10.1093/oxfordjournals.jbchem.a124642

DO - 10.1093/oxfordjournals.jbchem.a124642

M3 - Article

C2 - 7896746

AN - SCOPUS:0028171443

VL - 116

SP - 1153

EP - 1155

JO - Journal of Biochemistry

JF - Journal of Biochemistry

SN - 0021-924X

IS - 5

ER -