1H, 15N, and 13C resonance assignments of Staphylococcus aureus extracellular adherence protein domain 4

Jordan L. Woehl, Daisuke Takahashi, Alvaro I. Herrera, Brian V. Geisbrecht, Om Prakash

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

The pathogenic bacterium Staphylococcus aureus has evolved to actively evade many aspects of the human innate immune system by expressing a series of secreted inhibitory proteins. Among these, the extracellular adherence protein (Eap) has been shown to inhibit the classical and lectin pathways of the complement system. By binding to complement component C4b, Eap is able to inhibit formation of the CP/LP C3 pro-convertase. Secreted full-length, mature Eap consists of four ~98 residue domains, all of which adopt a similar beta-grasp fold, and are connected through a short linker region. Through multiple biochemical approaches, it has been determined that the third and fourth domains of Eap are responsible for C4b binding. Here we report the backbone and side-chain resonance assignments of the 11.3 kDa fourth domain of Eap. The assignment data has been deposited in the BMRB database under the accession number 26726.

元の言語英語
ページ(範囲)301-305
ページ数5
ジャーナルBiomolecular NMR Assignments
10
発行部数2
DOI
出版物ステータス出版済み - 10 1 2016
外部発表Yes

Fingerprint

Proteins
Complement C4b
Mannose-Binding Lectin Complement Pathway
Complement C4b-Binding Protein
Complement C3-C5 Convertases
Classical Complement Pathway
Complement C3
Immune system
Hand Strength
Lectins
Staphylococcus aureus
Immune System
Bacteria
Staphylococcus aureus Eap-N protein
Protein Domains
Databases

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry

これを引用

1H, 15N, and 13C resonance assignments of Staphylococcus aureus extracellular adherence protein domain 4. / Woehl, Jordan L.; Takahashi, Daisuke; Herrera, Alvaro I.; Geisbrecht, Brian V.; Prakash, Om.

:: Biomolecular NMR Assignments, 巻 10, 番号 2, 01.10.2016, p. 301-305.

研究成果: ジャーナルへの寄稿記事

Woehl, Jordan L. ; Takahashi, Daisuke ; Herrera, Alvaro I. ; Geisbrecht, Brian V. ; Prakash, Om. / 1H, 15N, and 13C resonance assignments of Staphylococcus aureus extracellular adherence protein domain 4. :: Biomolecular NMR Assignments. 2016 ; 巻 10, 番号 2. pp. 301-305.
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