Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent

Meng Tsen Ke, Yasuhiro Nakai, Satoshi Fujimoto, Rie Takayama, Shuhei Yoshida, Tomoya S. Kitajima, Makoto Sato, Takeshi Imai

研究成果: ジャーナルへの寄稿記事

69 引用 (Scopus)

抄録

Super-resolution imaging deep inside tissues has been challenging, as it is extremely sensitive to light scattering and spherical aberrations. Here, we report an optimized optical clearing agent for high-resolution fluorescence imaging (SeeDB2). SeeDB2 matches the refractive indices of fixed tissues to that of immersion oil (1.518), thus minimizing both light scattering and spherical aberrations. During the clearing process, fine morphology and fluorescent proteins were highly preserved. SeeDB2 enabled super-resolution microscopy of various tissue samples up to a depth of >100 μm, an order of magnitude deeper than previously possible under standard mounting conditions. Using this approach, we demonstrate accumulation of inhibitory synapses on spine heads in NMDA-receptor-deficient neurons. In the fly medulla, we found unexpected heterogeneity in axon bouton orientations among Mi1 neurons, a part of the motion detection circuitry. Thus, volumetric super-resolution microscopy of cleared tissues is a powerful strategy in connectomic studies at synaptic levels.

元の言語英語
ページ(範囲)2718-2732
ページ数15
ジャーナルCell Reports
14
発行部数11
DOI
出版物ステータス出版済み - 3 22 2016

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Tissue
Aberrations
Light scattering
Neurons
Microscopy
Microscopic examination
Connectome
Imaging techniques
Light
Refractometry
Optical Imaging
Immersion
N-Methyl-D-Aspartate Receptors
Mountings
Diptera
Synapses
Axons
Refractive index
Oils
Spine

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent. / Ke, Meng Tsen; Nakai, Yasuhiro; Fujimoto, Satoshi; Takayama, Rie; Yoshida, Shuhei; Kitajima, Tomoya S.; Sato, Makoto; Imai, Takeshi.

:: Cell Reports, 巻 14, 番号 11, 22.03.2016, p. 2718-2732.

研究成果: ジャーナルへの寄稿記事

Ke, MT, Nakai, Y, Fujimoto, S, Takayama, R, Yoshida, S, Kitajima, TS, Sato, M & Imai, T 2016, 'Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent', Cell Reports, 巻. 14, 番号 11, pp. 2718-2732. https://doi.org/10.1016/j.celrep.2016.02.057
Ke, Meng Tsen ; Nakai, Yasuhiro ; Fujimoto, Satoshi ; Takayama, Rie ; Yoshida, Shuhei ; Kitajima, Tomoya S. ; Sato, Makoto ; Imai, Takeshi. / Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent. :: Cell Reports. 2016 ; 巻 14, 番号 11. pp. 2718-2732.
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