Supercritical fluid chromatography/orbitrap mass spectrometry based lipidomics platform coupled with automated lipid identification software for accurate lipid profiling

Takayuki Yamada, Takato Uchikata, Shigeru Sakamoto, Yasuto Yokoi, Shin Nishiumi, Masaru Yoshida, Eiichiro Fukusaki, Takeshi Bamba

研究成果: ジャーナルへの寄稿学術誌査読

71 被引用数 (Scopus)

抄録

We developed a practical analytical system for high-throughput and comprehensive lipid profiling using a supercritical fluid chromatography (SFC) system coupled to an Orbitrap Fourier transform mass spectrometer (Orbitrap FT-MS). Using our SFC method, polar lipid molecular species were separated based on not only their fatty acyl moieties but also their polar head groups, using a single octadecylsilyl (ODS) column. In addition, because automatic data processing software was used for the identification of lipid molecular species, the analysis time including data processing was about a half an hour per sample. A variety of lipid molecular species were detected in mouse plasma, and isomers which often co-elute in reverse phase separation were identified accurately and quantified individually. To the best of our knowledge, this is the first report describing the chromatographic separation of lipids based on both fatty acyl moieties and polar head groups, using a single ODS column. Our results demonstrate that SFC/MS is a powerful tool for the simultaneous analysis of diverse lipid molecular species.

本文言語英語
ページ(範囲)237-242
ページ数6
ジャーナルJournal of Chromatography A
1301
DOI
出版ステータス出版済み - 8月 2 2013
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 分析化学
  • 生化学
  • 有機化学

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