Suppression of carbonic anhydrase III in rat liver by a dioxin-related toxic compound, coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl

Masumi Ikeda, Yuji Ishii, Harutoshi Kato, Daisuke Akazawa, Megumu Hatsumura, Takumi Ishida, Kimihiko Matsusue, Hideyuki Yamada, Kazuta Oguri

研究成果: ジャーナルへの寄稿学術誌査読

17 被引用数 (Scopus)

抄録

A coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl (PenCB), significantly suppresses the expression of rat liver carbonic anhydrase III (CAIII), an enzyme which has recently been suggested to prevent from H2O2-inducible apoptosis. Marked changes in the CAIII levels of liver cytosol were observed in rats following doses of PenCB ranging from 0.5 to 25 mg/kg body weight and maximum suppression was observed at a dose of 10 mg/kg. Northern analysis revealed that the level of CAIII mRNA in rat liver was dramatically reduced by PenCB treatment while only weak suppression was observed in pair-fed controls. Two AU-rich elements, considered as a destabilizing signal of mRNA, were found in the 3'-untranslated region of CAIII sequenced after reverse transcription-PCR and 3'-rapid amplification of the cDNA end. Dramatic decrease of CAIII in rat liver by PenCB could account for the suppression of the defense system for oxidative stress. (C) 2000 Academic Press.

本文言語英語
ページ(範囲)159-164
ページ数6
ジャーナルArchives of Biochemistry and Biophysics
380
1
DOI
出版ステータス出版済み - 8月 1 2000

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学

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