抄録
A coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl (PenCB), significantly suppresses the expression of rat liver carbonic anhydrase III (CAIII), an enzyme which has recently been suggested to prevent from H2O2-inducible apoptosis. Marked changes in the CAIII levels of liver cytosol were observed in rats following doses of PenCB ranging from 0.5 to 25 mg/kg body weight and maximum suppression was observed at a dose of 10 mg/kg. Northern analysis revealed that the level of CAIII mRNA in rat liver was dramatically reduced by PenCB treatment while only weak suppression was observed in pair-fed controls. Two AU-rich elements, considered as a destabilizing signal of mRNA, were found in the 3'-untranslated region of CAIII sequenced after reverse transcription-PCR and 3'-rapid amplification of the cDNA end. Dramatic decrease of CAIII in rat liver by PenCB could account for the suppression of the defense system for oxidative stress. (C) 2000 Academic Press.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 159-164 |
| ページ数 | 6 |
| ジャーナル | Archives of Biochemistry and Biophysics |
| 巻 | 380 |
| 発行部数 | 1 |
| DOI | |
| 出版物ステータス | 出版済み - 8 1 2000 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
これを引用
Suppression of carbonic anhydrase III in rat liver by a dioxin-related toxic compound, coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl. / Ikeda, Masumi; Ishii, Yuji; Kato, Harutoshi; Akazawa, Daisuke; Hatsumura, Megumu; Ishida, Takumi; Matsusue, Kimihiko; Yamada, Hideyuki; Oguri, Kazuta.
：: Archives of Biochemistry and Biophysics, 巻 380, 番号 1, 01.08.2000, p. 159-164.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Suppression of carbonic anhydrase III in rat liver by a dioxin-related toxic compound, coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl
AU - Ikeda, Masumi
AU - Ishii, Yuji
AU - Kato, Harutoshi
AU - Akazawa, Daisuke
AU - Hatsumura, Megumu
AU - Ishida, Takumi
AU - Matsusue, Kimihiko
AU - Yamada, Hideyuki
AU - Oguri, Kazuta
PY - 2000/8/1
Y1 - 2000/8/1
N2 - A coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl (PenCB), significantly suppresses the expression of rat liver carbonic anhydrase III (CAIII), an enzyme which has recently been suggested to prevent from H2O2-inducible apoptosis. Marked changes in the CAIII levels of liver cytosol were observed in rats following doses of PenCB ranging from 0.5 to 25 mg/kg body weight and maximum suppression was observed at a dose of 10 mg/kg. Northern analysis revealed that the level of CAIII mRNA in rat liver was dramatically reduced by PenCB treatment while only weak suppression was observed in pair-fed controls. Two AU-rich elements, considered as a destabilizing signal of mRNA, were found in the 3'-untranslated region of CAIII sequenced after reverse transcription-PCR and 3'-rapid amplification of the cDNA end. Dramatic decrease of CAIII in rat liver by PenCB could account for the suppression of the defense system for oxidative stress. (C) 2000 Academic Press.
AB - A coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl (PenCB), significantly suppresses the expression of rat liver carbonic anhydrase III (CAIII), an enzyme which has recently been suggested to prevent from H2O2-inducible apoptosis. Marked changes in the CAIII levels of liver cytosol were observed in rats following doses of PenCB ranging from 0.5 to 25 mg/kg body weight and maximum suppression was observed at a dose of 10 mg/kg. Northern analysis revealed that the level of CAIII mRNA in rat liver was dramatically reduced by PenCB treatment while only weak suppression was observed in pair-fed controls. Two AU-rich elements, considered as a destabilizing signal of mRNA, were found in the 3'-untranslated region of CAIII sequenced after reverse transcription-PCR and 3'-rapid amplification of the cDNA end. Dramatic decrease of CAIII in rat liver by PenCB could account for the suppression of the defense system for oxidative stress. (C) 2000 Academic Press.
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UR - http://www.scopus.com/inward/citedby.url?scp=0034254593&partnerID=8YFLogxK
U2 - 10.1006/abbi.2000.1911
DO - 10.1006/abbi.2000.1911
M3 - Article
C2 - 10900145
AN - SCOPUS:0034254593
VL - 380
SP - 159
EP - 164
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
IS - 1
ER -