Suppression of neural stem cell differentiation by Honeycomb-patterned films

Akinori Tsuruma, Masaru Tanaka, Sada Aki Yamamoto, Masatsugu Shimomura

研究成果: Contribution to journalConference article査読


Neural stem cells (NSCs) play important roles in brain development. NSCs have self-renewal potency and can differentiate into phenotypes of neural cells (neuron, astrocyte and oligodendrocyte). Recently, NSCs are expected for regenerative medicine to be transplanted into damaged neural tissues. For this purpose, it is important to maintain these properties of NSCs in culture. Nano-micro patterns on the surface of scaffold have significant influences on the cell morphologies, proliferation, differentiation and function. In previous study, we prepared highly regular porous films (honeycomb-patterned films) by a casting technique, and investigated neural cell morphologies on flat film and honeycomb-patterned films. As a result, morphologies of adhered neural cells were changed by varying pore size of the honeycomb-patterned films. Especially, we found that cell aggregates were formed on the patterned films of pore size 3 m. Our current study showed that NSCs were suppressed its differentiation into neurons on the patterned films of pore size 3 μm. This result indicate that NSCs are positively regulated their own survival and differentiation. To use neural stem cells / progenitor cells for regenerative medicine, we need environments where these NSCs can live in vitro without losing their ability to proliferation and differentiation.

ジャーナルIFMBE Proceedings
出版ステータス出版済み - 1 1 2007
イベント10th World Congress on Medical Physics and Biomedical Engineering, WC 2006 - Seoul, 大韓民国
継続期間: 8 27 20069 1 2006

All Science Journal Classification (ASJC) codes

  • バイオエンジニアリング
  • 生体医工学


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