Suppression of superoxide anion production by interleukin-10 is accompanied by a downregulation of the genes for subunit proteins of NADPH oxidase

S. Kuga, T. Otsuka, H. Niiro, H. Nunoi, Y. Nemoto, T. Nakano, T. Ogo, T. Umei, Y. Niho

研究成果: ジャーナルへの寄稿記事

58 引用 (Scopus)

抄録

Interleukin-10 (IL-10) inhibited the production of superoxide anion (O2-) by both unactivated and interferon-γ (IFN-γ)-activated human monocytes. Simultaneous addition of IL-10 with IFN-γ at the start of incubation was necessary for an optimal inhibitory effect. The degree of inhibition was substantially comparable to that of IL-4, and the combination of suboptimal concentrations of IL-10 and IL-4 produced an additive effect. A similar effect was also obtained when viral IL-10 (vIL-10) was used instead of IL-10. The inhibitory effect of IL-10 was accompanied by the reduced accumulation of transcripts for heavy chain subunit of cytochrome b558 (gp91-phox) and 47-kD cytosolic factor (p47-phox), components of the O2--generating NADPH oxidase system. Reduction of the mRNAs was distinct within 24 hours. On the other hand, the induced O2- production by human monocytic leukemia cell lines (THP-1 and HL60) was not inhibited by IL-10. The amount of gp91-phox and p47-phox mRNAs remained unchanged even in the presence of excess amount of IL-10. Taken together, these results suggest that IL-10 inhibits O2- production by downregulation of the gp91-phox and p47-phox genes in human monocytes.

元の言語英語
ページ(範囲)151-157
ページ数7
ジャーナルExperimental Hematology
24
発行部数2
出版物ステータス出版済み - 3 20 1996

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NADPH Oxidase
Superoxides
Interleukin-10
Down-Regulation
Proteins
Interleukin-4
Interferons
Monocytes
Messenger RNA
4-ethoxymethylene-2-phenyl-2-oxazoline-5-one
Leukemia
Cell Line

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

これを引用

Suppression of superoxide anion production by interleukin-10 is accompanied by a downregulation of the genes for subunit proteins of NADPH oxidase. / Kuga, S.; Otsuka, T.; Niiro, H.; Nunoi, H.; Nemoto, Y.; Nakano, T.; Ogo, T.; Umei, T.; Niho, Y.

:: Experimental Hematology, 巻 24, 番号 2, 20.03.1996, p. 151-157.

研究成果: ジャーナルへの寄稿記事

Kuga, S, Otsuka, T, Niiro, H, Nunoi, H, Nemoto, Y, Nakano, T, Ogo, T, Umei, T & Niho, Y 1996, 'Suppression of superoxide anion production by interleukin-10 is accompanied by a downregulation of the genes for subunit proteins of NADPH oxidase', Experimental Hematology, 巻. 24, 番号 2, pp. 151-157.
Kuga, S. ; Otsuka, T. ; Niiro, H. ; Nunoi, H. ; Nemoto, Y. ; Nakano, T. ; Ogo, T. ; Umei, T. ; Niho, Y. / Suppression of superoxide anion production by interleukin-10 is accompanied by a downregulation of the genes for subunit proteins of NADPH oxidase. :: Experimental Hematology. 1996 ; 巻 24, 番号 2. pp. 151-157.
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abstract = "Interleukin-10 (IL-10) inhibited the production of superoxide anion (O2-) by both unactivated and interferon-γ (IFN-γ)-activated human monocytes. Simultaneous addition of IL-10 with IFN-γ at the start of incubation was necessary for an optimal inhibitory effect. The degree of inhibition was substantially comparable to that of IL-4, and the combination of suboptimal concentrations of IL-10 and IL-4 produced an additive effect. A similar effect was also obtained when viral IL-10 (vIL-10) was used instead of IL-10. The inhibitory effect of IL-10 was accompanied by the reduced accumulation of transcripts for heavy chain subunit of cytochrome b558 (gp91-phox) and 47-kD cytosolic factor (p47-phox), components of the O2--generating NADPH oxidase system. Reduction of the mRNAs was distinct within 24 hours. On the other hand, the induced O2- production by human monocytic leukemia cell lines (THP-1 and HL60) was not inhibited by IL-10. The amount of gp91-phox and p47-phox mRNAs remained unchanged even in the presence of excess amount of IL-10. Taken together, these results suggest that IL-10 inhibits O2- production by downregulation of the gp91-phox and p47-phox genes in human monocytes.",
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T1 - Suppression of superoxide anion production by interleukin-10 is accompanied by a downregulation of the genes for subunit proteins of NADPH oxidase

AU - Kuga, S.

AU - Otsuka, T.

AU - Niiro, H.

AU - Nunoi, H.

AU - Nemoto, Y.

AU - Nakano, T.

AU - Ogo, T.

AU - Umei, T.

AU - Niho, Y.

PY - 1996/3/20

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N2 - Interleukin-10 (IL-10) inhibited the production of superoxide anion (O2-) by both unactivated and interferon-γ (IFN-γ)-activated human monocytes. Simultaneous addition of IL-10 with IFN-γ at the start of incubation was necessary for an optimal inhibitory effect. The degree of inhibition was substantially comparable to that of IL-4, and the combination of suboptimal concentrations of IL-10 and IL-4 produced an additive effect. A similar effect was also obtained when viral IL-10 (vIL-10) was used instead of IL-10. The inhibitory effect of IL-10 was accompanied by the reduced accumulation of transcripts for heavy chain subunit of cytochrome b558 (gp91-phox) and 47-kD cytosolic factor (p47-phox), components of the O2--generating NADPH oxidase system. Reduction of the mRNAs was distinct within 24 hours. On the other hand, the induced O2- production by human monocytic leukemia cell lines (THP-1 and HL60) was not inhibited by IL-10. The amount of gp91-phox and p47-phox mRNAs remained unchanged even in the presence of excess amount of IL-10. Taken together, these results suggest that IL-10 inhibits O2- production by downregulation of the gp91-phox and p47-phox genes in human monocytes.

AB - Interleukin-10 (IL-10) inhibited the production of superoxide anion (O2-) by both unactivated and interferon-γ (IFN-γ)-activated human monocytes. Simultaneous addition of IL-10 with IFN-γ at the start of incubation was necessary for an optimal inhibitory effect. The degree of inhibition was substantially comparable to that of IL-4, and the combination of suboptimal concentrations of IL-10 and IL-4 produced an additive effect. A similar effect was also obtained when viral IL-10 (vIL-10) was used instead of IL-10. The inhibitory effect of IL-10 was accompanied by the reduced accumulation of transcripts for heavy chain subunit of cytochrome b558 (gp91-phox) and 47-kD cytosolic factor (p47-phox), components of the O2--generating NADPH oxidase system. Reduction of the mRNAs was distinct within 24 hours. On the other hand, the induced O2- production by human monocytic leukemia cell lines (THP-1 and HL60) was not inhibited by IL-10. The amount of gp91-phox and p47-phox mRNAs remained unchanged even in the presence of excess amount of IL-10. Taken together, these results suggest that IL-10 inhibits O2- production by downregulation of the gp91-phox and p47-phox genes in human monocytes.

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