Suppressive effect of methylsulfonylmethane (MSM) on type II collagen-induced arthritis in DBA/1J mice

Takashi Hasegawa, Sugi Ueno, Shoichiro Kumamoto, Yasunobu Yoshikai

    研究成果: ジャーナルへの寄稿記事

    16 引用 (Scopus)

    抄録

    Background: Methylsulfonylmethane (MSM) is a natural constituent of the environment found in plants, cow's milk and urine of both bovines and humans. It has been reported that MSM was efficacious in the reduction of symptoms associated with seasonal allergic rhinitis, and protected from the development of murine autoimmune lymphoproliferative symptoms in MRL/lpr mice and the destructive changes in the joints of MRL/Mn/lnr female mice with spontaneous arthritis. In this study, we investigated the effect of MSM on type II collagen-induced murine arthritis as an animal model of rheumatoid arthritis. Methods: Murine arthritis was induced as follows. Male DBA/1J mice were injected intradermally at the base of the tail with 200 μg of the type II collagen. Three weeks after primary immunization, the mice were boosted in the same way. Male DBA/1J mice were placed on a continuous treatment regimen with 2.5% MSM in the drinking water, ad libitum, commencing at one week before primary immunization of the type II collagen. The clinical severity of arthritis (deformation and swelling) was scored based on the appearance of each paw which was graded scale. Results: The arithritic score (deformation) and swelling score increased gradually from two weeks up to eight weeks after the type II collagen immunization. On the other hand, the arithritic score in the MSM drinking mice was significantly lower than that in control mice. The total number of leukocytes in inguinal lymph nodes was significantly smaller in the MSM drinking mice than that in control mice. Flowcytometry revealed that the number of B220 + cells in the lymph nodes was significantly smaller in the MSM drinking mice than that in control mice. Notably, expression level of IL-12 p40 mRNA was reduced in spleen of the MSM drinking mice as compared with control mice Conclusion: These results suggested that MSM administration was able to modify the immune responses to the type II collagen, resulting in protection of the development of artiritis of type II collagen induced arthritis in DBA/1J mice.

    元の言語英語
    ページ(範囲)421-427
    ページ数7
    ジャーナルJapanese Pharmacology and Therapeutics
    32
    発行部数7
    出版物ステータス出版済み - 8 31 2004

    Fingerprint

    Inbred DBA Mouse
    Experimental Arthritis
    Collagen Type II
    Drinking
    Arthritis
    Immunization
    Lymph Nodes
    Inbred MRL lpr Mouse
    dimethyl sulfone
    Seasonal Allergic Rhinitis
    Groin
    Interleukin-12
    Leukocyte Count
    Drinking Water
    Tail
    Rheumatoid Arthritis
    Milk
    Spleen
    Animal Models
    Cell Count

    All Science Journal Classification (ASJC) codes

    • Pharmacology
    • Pharmacology (medical)

    これを引用

    Suppressive effect of methylsulfonylmethane (MSM) on type II collagen-induced arthritis in DBA/1J mice. / Hasegawa, Takashi; Ueno, Sugi; Kumamoto, Shoichiro; Yoshikai, Yasunobu.

    :: Japanese Pharmacology and Therapeutics, 巻 32, 番号 7, 31.08.2004, p. 421-427.

    研究成果: ジャーナルへの寄稿記事

    Hasegawa, Takashi ; Ueno, Sugi ; Kumamoto, Shoichiro ; Yoshikai, Yasunobu. / Suppressive effect of methylsulfonylmethane (MSM) on type II collagen-induced arthritis in DBA/1J mice. :: Japanese Pharmacology and Therapeutics. 2004 ; 巻 32, 番号 7. pp. 421-427.
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    title = "Suppressive effect of methylsulfonylmethane (MSM) on type II collagen-induced arthritis in DBA/1J mice",
    abstract = "Background: Methylsulfonylmethane (MSM) is a natural constituent of the environment found in plants, cow's milk and urine of both bovines and humans. It has been reported that MSM was efficacious in the reduction of symptoms associated with seasonal allergic rhinitis, and protected from the development of murine autoimmune lymphoproliferative symptoms in MRL/lpr mice and the destructive changes in the joints of MRL/Mn/lnr female mice with spontaneous arthritis. In this study, we investigated the effect of MSM on type II collagen-induced murine arthritis as an animal model of rheumatoid arthritis. Methods: Murine arthritis was induced as follows. Male DBA/1J mice were injected intradermally at the base of the tail with 200 μg of the type II collagen. Three weeks after primary immunization, the mice were boosted in the same way. Male DBA/1J mice were placed on a continuous treatment regimen with 2.5{\%} MSM in the drinking water, ad libitum, commencing at one week before primary immunization of the type II collagen. The clinical severity of arthritis (deformation and swelling) was scored based on the appearance of each paw which was graded scale. Results: The arithritic score (deformation) and swelling score increased gradually from two weeks up to eight weeks after the type II collagen immunization. On the other hand, the arithritic score in the MSM drinking mice was significantly lower than that in control mice. The total number of leukocytes in inguinal lymph nodes was significantly smaller in the MSM drinking mice than that in control mice. Flowcytometry revealed that the number of B220 + cells in the lymph nodes was significantly smaller in the MSM drinking mice than that in control mice. Notably, expression level of IL-12 p40 mRNA was reduced in spleen of the MSM drinking mice as compared with control mice Conclusion: These results suggested that MSM administration was able to modify the immune responses to the type II collagen, resulting in protection of the development of artiritis of type II collagen induced arthritis in DBA/1J mice.",
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    T1 - Suppressive effect of methylsulfonylmethane (MSM) on type II collagen-induced arthritis in DBA/1J mice

    AU - Hasegawa, Takashi

    AU - Ueno, Sugi

    AU - Kumamoto, Shoichiro

    AU - Yoshikai, Yasunobu

    PY - 2004/8/31

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    N2 - Background: Methylsulfonylmethane (MSM) is a natural constituent of the environment found in plants, cow's milk and urine of both bovines and humans. It has been reported that MSM was efficacious in the reduction of symptoms associated with seasonal allergic rhinitis, and protected from the development of murine autoimmune lymphoproliferative symptoms in MRL/lpr mice and the destructive changes in the joints of MRL/Mn/lnr female mice with spontaneous arthritis. In this study, we investigated the effect of MSM on type II collagen-induced murine arthritis as an animal model of rheumatoid arthritis. Methods: Murine arthritis was induced as follows. Male DBA/1J mice were injected intradermally at the base of the tail with 200 μg of the type II collagen. Three weeks after primary immunization, the mice were boosted in the same way. Male DBA/1J mice were placed on a continuous treatment regimen with 2.5% MSM in the drinking water, ad libitum, commencing at one week before primary immunization of the type II collagen. The clinical severity of arthritis (deformation and swelling) was scored based on the appearance of each paw which was graded scale. Results: The arithritic score (deformation) and swelling score increased gradually from two weeks up to eight weeks after the type II collagen immunization. On the other hand, the arithritic score in the MSM drinking mice was significantly lower than that in control mice. The total number of leukocytes in inguinal lymph nodes was significantly smaller in the MSM drinking mice than that in control mice. Flowcytometry revealed that the number of B220 + cells in the lymph nodes was significantly smaller in the MSM drinking mice than that in control mice. Notably, expression level of IL-12 p40 mRNA was reduced in spleen of the MSM drinking mice as compared with control mice Conclusion: These results suggested that MSM administration was able to modify the immune responses to the type II collagen, resulting in protection of the development of artiritis of type II collagen induced arthritis in DBA/1J mice.

    AB - Background: Methylsulfonylmethane (MSM) is a natural constituent of the environment found in plants, cow's milk and urine of both bovines and humans. It has been reported that MSM was efficacious in the reduction of symptoms associated with seasonal allergic rhinitis, and protected from the development of murine autoimmune lymphoproliferative symptoms in MRL/lpr mice and the destructive changes in the joints of MRL/Mn/lnr female mice with spontaneous arthritis. In this study, we investigated the effect of MSM on type II collagen-induced murine arthritis as an animal model of rheumatoid arthritis. Methods: Murine arthritis was induced as follows. Male DBA/1J mice were injected intradermally at the base of the tail with 200 μg of the type II collagen. Three weeks after primary immunization, the mice were boosted in the same way. Male DBA/1J mice were placed on a continuous treatment regimen with 2.5% MSM in the drinking water, ad libitum, commencing at one week before primary immunization of the type II collagen. The clinical severity of arthritis (deformation and swelling) was scored based on the appearance of each paw which was graded scale. Results: The arithritic score (deformation) and swelling score increased gradually from two weeks up to eight weeks after the type II collagen immunization. On the other hand, the arithritic score in the MSM drinking mice was significantly lower than that in control mice. The total number of leukocytes in inguinal lymph nodes was significantly smaller in the MSM drinking mice than that in control mice. Flowcytometry revealed that the number of B220 + cells in the lymph nodes was significantly smaller in the MSM drinking mice than that in control mice. Notably, expression level of IL-12 p40 mRNA was reduced in spleen of the MSM drinking mice as compared with control mice Conclusion: These results suggested that MSM administration was able to modify the immune responses to the type II collagen, resulting in protection of the development of artiritis of type II collagen induced arthritis in DBA/1J mice.

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