Surface adhesion of salmonella enterica and staphylococcus aureus under fluctuating temperatures

D. Hamanaka, K. Morimatsu, F. Tanaka, T. Uchino

研究成果: Chapter in Book/Report/Conference proceedingChapter

抄録

The bacterial adherent characteristics of two pathogens (Salmonella enterica, Staphylococcus aureus) on the surface of polystyrene were investigated under a fluctuating temperature regime and different nutrient levels (trypticase soy broth). Bacterial adhesions were evaluated by the optical density value of bacterial cells stained with 0.1% (w/v) of crystal violet solution. Under constant temperature conditions at 5 and 30°C, the adherent potentials of both S. enterica and S. aureus were stable at the end of incubation (5 days). Under a fluctuating temperature regime, the cell adhesion potentials were changed with the period of incubation. The adhesions of both pathogens were considerably increased by rapid temperature elevation from 5 to 30°C, while approximately twice the optical density value of cell adhesion of S. enterica was observed on the polystyrene surface compared with constant conditions. Transferring the sample tube to 5°C after fluctuation from 5 to 30°C led to considerable cell detachment of S. enterica by the end of incubation. It was probable that S. enterica cells used in this study was extremely sensitive to environmental variation, consequently cell adhesion and release could be observed under fluctuating temperature conditions. Meanwhile, stable adhesion of S. aureus cells was observed at 5°C after temperature fluctuations from 5 to 30°C.

本文言語英語
ホスト出版物のタイトルInternational Symposium Postharvest Pacifica 2009 - Pathways to Quality
ホスト出版物のサブタイトルV International Symposium on Managing Quality in Chains + Australasian Postharvest Horticultural Conference
出版社International Society for Horticultural Science
ページ541-546
ページ数6
ISBN(印刷版)9789066056039
DOI
出版ステータス出版済み - 11 25 2010

出版物シリーズ

名前Acta Horticulturae
880
ISSN(印刷版)0567-7572

All Science Journal Classification (ASJC) codes

  • 園芸学

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