The bacterial adherent characteristics of two pathogens (Salmonella enterica, Staphylococcus aureus) on the surface of polystyrene were investigated under a fluctuating temperature regime and different nutrient levels (trypticase soy broth). Bacterial adhesions were evaluated by the optical density value of bacterial cells stained with 0.1% (w/v) of crystal violet solution. Under constant temperature conditions at 5 and 30°C, the adherent potentials of both S. enterica and S. aureus were stable at the end of incubation (5 days). Under a fluctuating temperature regime, the cell adhesion potentials were changed with the period of incubation. The adhesions of both pathogens were considerably increased by rapid temperature elevation from 5 to 30°C, while approximately twice the optical density value of cell adhesion of S. enterica was observed on the polystyrene surface compared with constant conditions. Transferring the sample tube to 5°C after fluctuation from 5 to 30°C led to considerable cell detachment of S. enterica by the end of incubation. It was probable that S. enterica cells used in this study was extremely sensitive to environmental variation, consequently cell adhesion and release could be observed under fluctuating temperature conditions. Meanwhile, stable adhesion of S. aureus cells was observed at 5°C after temperature fluctuations from 5 to 30°C.