Surface modification of a solid-state cellulose matrix with lactose by a surfactant-enveloped enzyme in a nonaqueous medium

Shizuka Egusa, Shingo Yokota, Kyoko Tanaka, Kei Esaki, Yuri Okutani, Yukiko Ogawa, Takuya Kitaoka, Masahiro Goto, Hiroyuki Wariishi

研究成果: ジャーナルへの寄稿学術誌査読

33 被引用数 (Scopus)

抄録

Glyco-modification of a solid cellulose surface with lactose via an enzymatic reaction was successfully achieved using a surfactant-enveloped enzyme (SEE) in a lithium chloride/dimethylacetamide solvent system. The unique biocatalyst, SEE, which is active in organic media, was prepared by protecting the surface of cellulase with the specific nonionic surfactant dioleyl-N-D-glucona-L-glutamate. Lactose, a second cellulase substrate, was introduced onto cellulose surfaces via SEE-mediated enzymatic reaction. Fluorescent labeling and imaging revealed the presence of galactose residues from lactose on the modified cellulose surface. X-Ray diffractometry showed that the crystal structure of cellulose remained unchanged even after glyco-modification. Cell adhesion assays were carried out using rat liver cells on which galactose-specific receptors are present, and the initial cell attachment (within 12 h) on the lactose-modified cellulose filter was greater than that on an original (unmodified) cellulose matrix. The SEE-mediated biocatalysis enabled efficient glyco-modification of the solid cellulose surface in a one-step reaction, without complicated pre-treatment of the cellulose matrix and donor sugar. Consequently, this simple and effective approach to surface modification of a solid cellulose matrix with bio-functional sugars would be expected to have wide potential applications in glycomaterials engineering.

本文言語英語
ページ(範囲)1836-1842
ページ数7
ジャーナルJournal of Materials Chemistry
19
13
DOI
出版ステータス出版済み - 2009

!!!All Science Journal Classification (ASJC) codes

  • 化学 (全般)
  • 材料化学

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