Surface plasmon resonance and surface plasmon field-enhanced fluorescence spectroscopy for sensitive detection of tumor markers.

Yusuke Arima, Yuji Teramura, Hiromi Takiguchi, Keiko Kawano, Hidetoshi Kotera, Hiroo Iwata

研究成果: Contribution to journalReview article査読

21 被引用数 (Scopus)

抄録

Surface plasmon resonance (SPR), which provides real-time, in situ analysis of dynamic surface events, is a valuable tool for studying interactions between biomolecules. In the clinical diagnosis of tumor markers in human blood, SPR is applied to detect the formation of a sandwich-type immune complex composed of a primary antibody immobilized on a sensor surface, the tumor marker, and a secondary antibody. However, the SPR signal is quite low due to the minute amounts (ng-pg/mL) of most tumor markers in blood. We have shown that the SPR signal can be amplified by applying an antibody against the secondary antibody or streptavidin-conjugated nanobeads that specifically accumulate on the secondary antibody. Another method employed for highly sensitive detection is the surface plasmon field-enhanced fluorescence spectroscopy-based immunoassay, which utilizes the enhanced electric field intensity at a metal/water interface to excite a fluorophore. Fluorescence intensity attributed to binding of a fluorophore-labeled secondary antibody is increased due to the enhanced field in the SPR condition and can be monitored in real time.

本文言語英語
ページ(範囲)3-20
ページ数18
ジャーナルMethods in molecular biology (Clifton, N.J.)
503
DOI
出版ステータス出版済み - 1 1 2009
外部発表はい

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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