Surface plasmon resonance-based highly sensitive immunosensing for brain natriuretic peptide using nanobeads for signal amplification

Yuji Teramura, Yusuke Arima, Hiroo Iwata

研究成果: ジャーナルへの寄稿学術誌査読

81 被引用数 (Scopus)

抄録

In this study, we developed a surface plasmon resonance (SPR)-based sensing method with high sensitivity for the detection of brain natriuretic peptide (BNP), the level of which is a useful indicator of heart failure. In our system, BNP was detected by a sandwich-type immunoassay using two kinds of monoclonal antibodies: primary and secondary. The SPR signal was amplified by using streptavidin-conjugated nanobeads approximately 50 nm in diameter that specifically accumulated on the secondary antibody via biotin-avidin bonding. By this method, the SPR signals were highly intensified, and thus picogram levels (pg/ml) of BNP could be detected. The streptavidin nanobead amplification method realizes detection of very low levels of BNP, as is necessary for the clinical diagnosis. It is also expected that our streptavidin nanobead amplification method can be applicable to the detection of other hormones and tumor markers that are present in only low concentrations within the human body.

本文言語英語
ページ(範囲)208-215
ページ数8
ジャーナルAnalytical Biochemistry
357
2
DOI
出版ステータス出版済み - 10月 15 2006
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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