Surface plasmon resonance imaging measurements of caspase reactions on peptide microarrays

Yusuke Inoue, Takeshi Mori, Go Yamanouchi, Xiaoming Han, Tatsuhiko Sonoda, Takuro Niidome, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

20 引用 (Scopus)

抄録

Enzymatic activity monitoring of caspases, which are a class of cysteine protease, was performed by using peptide arrays based on surface plasmon resonance (SPR) imaging. The strategy of the detection is straightforward, using streptavidin to amplify the SPR signals of the surface-immobilized substrate peptides labeled with biotin at the C termini. Thus, the cleavage of the substrate peptides by caspases was detected as a signal decrease. Using this method, we succeeded in monitoring the activities of purified caspases and caspases in cell lysates. The SPR imaging-based peptide array would be applicable to cell-based drug screening and biochemical studies to reveal signal transduction processes.

元の言語英語
ページ(範囲)147-149
ページ数3
ジャーナルAnalytical Biochemistry
375
発行部数1
DOI
出版物ステータス出版済み - 4 1 2008

Fingerprint

Surface Plasmon Resonance
Surface plasmon resonance
Microarrays
Caspases
Imaging techniques
Peptides
Signal transduction
Preclinical Drug Evaluations
Streptavidin
Cysteine Proteases
Monitoring
Substrates
Biotin
Signal Transduction
Screening
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

これを引用

Surface plasmon resonance imaging measurements of caspase reactions on peptide microarrays. / Inoue, Yusuke; Mori, Takeshi; Yamanouchi, Go; Han, Xiaoming; Sonoda, Tatsuhiko; Niidome, Takuro; Katayama, Yoshiki.

:: Analytical Biochemistry, 巻 375, 番号 1, 01.04.2008, p. 147-149.

研究成果: ジャーナルへの寄稿記事

Inoue, Yusuke ; Mori, Takeshi ; Yamanouchi, Go ; Han, Xiaoming ; Sonoda, Tatsuhiko ; Niidome, Takuro ; Katayama, Yoshiki. / Surface plasmon resonance imaging measurements of caspase reactions on peptide microarrays. :: Analytical Biochemistry. 2008 ; 巻 375, 番号 1. pp. 147-149.
@article{df24550707f6416fa1b92fb4cc0aaf52,
title = "Surface plasmon resonance imaging measurements of caspase reactions on peptide microarrays",
abstract = "Enzymatic activity monitoring of caspases, which are a class of cysteine protease, was performed by using peptide arrays based on surface plasmon resonance (SPR) imaging. The strategy of the detection is straightforward, using streptavidin to amplify the SPR signals of the surface-immobilized substrate peptides labeled with biotin at the C termini. Thus, the cleavage of the substrate peptides by caspases was detected as a signal decrease. Using this method, we succeeded in monitoring the activities of purified caspases and caspases in cell lysates. The SPR imaging-based peptide array would be applicable to cell-based drug screening and biochemical studies to reveal signal transduction processes.",
author = "Yusuke Inoue and Takeshi Mori and Go Yamanouchi and Xiaoming Han and Tatsuhiko Sonoda and Takuro Niidome and Yoshiki Katayama",
year = "2008",
month = "4",
day = "1",
doi = "10.1016/j.ab.2007.11.040",
language = "English",
volume = "375",
pages = "147--149",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Surface plasmon resonance imaging measurements of caspase reactions on peptide microarrays

AU - Inoue, Yusuke

AU - Mori, Takeshi

AU - Yamanouchi, Go

AU - Han, Xiaoming

AU - Sonoda, Tatsuhiko

AU - Niidome, Takuro

AU - Katayama, Yoshiki

PY - 2008/4/1

Y1 - 2008/4/1

N2 - Enzymatic activity monitoring of caspases, which are a class of cysteine protease, was performed by using peptide arrays based on surface plasmon resonance (SPR) imaging. The strategy of the detection is straightforward, using streptavidin to amplify the SPR signals of the surface-immobilized substrate peptides labeled with biotin at the C termini. Thus, the cleavage of the substrate peptides by caspases was detected as a signal decrease. Using this method, we succeeded in monitoring the activities of purified caspases and caspases in cell lysates. The SPR imaging-based peptide array would be applicable to cell-based drug screening and biochemical studies to reveal signal transduction processes.

AB - Enzymatic activity monitoring of caspases, which are a class of cysteine protease, was performed by using peptide arrays based on surface plasmon resonance (SPR) imaging. The strategy of the detection is straightforward, using streptavidin to amplify the SPR signals of the surface-immobilized substrate peptides labeled with biotin at the C termini. Thus, the cleavage of the substrate peptides by caspases was detected as a signal decrease. Using this method, we succeeded in monitoring the activities of purified caspases and caspases in cell lysates. The SPR imaging-based peptide array would be applicable to cell-based drug screening and biochemical studies to reveal signal transduction processes.

UR - http://www.scopus.com/inward/record.url?scp=39749179110&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39749179110&partnerID=8YFLogxK

U2 - 10.1016/j.ab.2007.11.040

DO - 10.1016/j.ab.2007.11.040

M3 - Article

C2 - 18167303

AN - SCOPUS:39749179110

VL - 375

SP - 147

EP - 149

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -