Surface plasmon resonance imaging measurements of caspase reactions on peptide microarrays

Yusuke Inoue, Takeshi Mori, Go Yamanouchi, Xiaoming Han, Tatsuhiko Sonoda, Takuro Niidome, Yoshiki Katayama

    研究成果: Contribution to journalArticle査読

    20 被引用数 (Scopus)

    抄録

    Enzymatic activity monitoring of caspases, which are a class of cysteine protease, was performed by using peptide arrays based on surface plasmon resonance (SPR) imaging. The strategy of the detection is straightforward, using streptavidin to amplify the SPR signals of the surface-immobilized substrate peptides labeled with biotin at the C termini. Thus, the cleavage of the substrate peptides by caspases was detected as a signal decrease. Using this method, we succeeded in monitoring the activities of purified caspases and caspases in cell lysates. The SPR imaging-based peptide array would be applicable to cell-based drug screening and biochemical studies to reveal signal transduction processes.

    本文言語英語
    ページ(範囲)147-149
    ページ数3
    ジャーナルAnalytical Biochemistry
    375
    1
    DOI
    出版ステータス出版済み - 4 1 2008

    All Science Journal Classification (ASJC) codes

    • 生物理学
    • 生化学
    • 分子生物学
    • 細胞生物学

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