Surface structure and biocompatibility of demineralized dentin matrix granules soaked in a simulated body fluid

Toshiyuki Akazawa, Masaru Murata, Jun Hino, Futami Nagano, Tatsuhiro Shigyo, Takafumi Nomura, Hiroyuki Inano, Kohji Itabashi, Tohru Yamagishi, Katsuo Nakamura, Touru Takahashi, Shunji Iida, Haruhiko Kashiwazaki

研究成果: ジャーナルへの寄稿学術誌査読

12 被引用数 (Scopus)

抄録

Demineralized dentin matrix (DDM) granules with excellent biocompatibility were easily prepared using unnecessary human teeth by a new cooling-pulverizing and demineralizing technique. Extracted human teeth were pulverized together with saline ice at 12,000 rpm-rotation number of a ZrO 2 blade for 30 s in a ZrO 2 vessel. The pulverized granules exhibited the particle size distribution of 0.5-2 mm that was efficient for regeneration of alveolar bone. The (Ca/P) ratios of the granules were 1.60-1.66, which were close to the stoichiometric value of 1.67 for standard hydroxyapatite (HAp). Small amounts of Na + and Mg 2+ ions present at less than 1% were detected. The pulverized granules were dissolved with stirring under 500 rpm for 10-60 min in 2.0%-HNO 3 solutions to obtain partial or complete DDM granules. As the dissolution time increased, crystallinity of HAp phase lowered and asperity on surfaces of the granules became outstanding due to elution of mineral components. At the dissolution of 60 min, the pulverizing granules were completely demineralized and the weight decreased to about one-fifth. To improve surface activity of the DDM granules without denaturation of bone growth factors, the DDM granules were soaked at 309.5 K and pH 7.40 in a simulated body fluid (SBF). HAp microcrystals were gradually precipitated on surfaces of the DDM granules with increasing the soaking time. Different morphology of the precipitates was observed, depending on the demineralization situation of the pulverized granules. For the DDM with low dissolution efficiency of 42%, porous bone-like apatites at 24 h after the soaking and fiber-oriented aggregates at 144 h were recognized. The bioactive DDM granules were implanted into the subcutaneous tissues of the back region of rats. At 4 weeks after the implantation, bio-absorption by comparatively small amounts of multi-giant cells was recognized around the surface layers of DDM granules.

本文言語英語
ページ(範囲)51-55
ページ数5
ジャーナルApplied Surface Science
262
DOI
出版ステータス出版済み - 12月 1 2012
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 化学 (全般)
  • 凝縮系物理学
  • 物理学および天文学(全般)
  • 表面および界面
  • 表面、皮膜および薄膜

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