Recent studies show that during slow freezing of biological cells, the cells may be also injured by not only chemical damage but also mechanical damage induced by ice crystal compression. A new experimental procedure is developed to quantify cell destruction by deformation with two parallel surfaces. The viability of cells (prostatic carcinoma cells, 17.5 μm in mean diameter) is measured as a function of gap size ranging from 3.5 μm to 16.2 μm at 0°C, 23°C and 37°C. The viability at a smaller gap size is significantly lower at 37°C than at 23°C, while the difference between 0°C and 23°C is much smaller. This suggests that deformation damage is related to the deformation of the cytoskeleton rather than the mechanical properties of the lipid membrane.
All Science Journal Classification (ASJC) codes
- Biomedical Engineering
- Physiology (medical)