TY - JOUR
T1 - Switch circular DNA formed in cytokine-treated mouse splenocytes
T2 - Evidence for intramolecular DNA deletion in immunoglobulin class switching
AU - Matsuoka, Masao
AU - Yoshida, Kazuya
AU - Maeda, Toyoki
AU - Usuda, Sadakazu
AU - Sakano, Hitoshi
N1 - Funding Information:
We thank Richard Maki for critically reading the manuscript, Mattias Wabl for communicating data prior to publication, Wesley Dunnick and Richard Maki for Cn gene clones, and David Clayton for the murine mtDNA clone. We are grateful to Mei Lie Wong for her electron microscopic preparations and to Donald 0. Davis for his comments. We also thank C. Michael Samson and Jenny Wu for technical assistance. M. M. is a fellow of the Leukemia Society of America (M1045). This research was supported by grants from the National institutes of Health (Al-18790) and the American Cancer Society (IM-366). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 16 U.S.C. Section 1734 solely to indicate this fact.
PY - 1990/7/13
Y1 - 1990/7/13
N2 - We have characterized circular DNA in mouse splenocytes treated with the mitogen lipopolysaccharide (LPS) and various cytokines, including transforming growth factor β (TGF-β) and interleukin 4 (IL-4). Using probes of immunoglobulin heavy chain constant genes (CH), excision products of class switch recombination were identified. The majority of the clones contained the 3′ portion of the switch μ (Sμ) region and the 5′ portion of other switch regions. Some clones contained 3′-Sγ sequences instead of 3′-Sμ. This indicates that isotype switching may occur not only from Cμ, but also from one of the Cγ genes to other CH genes further downstream. In the presence of LPS, the cytokine TGF-β enhanced the detection of 5′-Sα-positive clones, while the lymphokine IL-4 enhanced 5′-Sγ1 positives. The data support the notion that TGF-β and IL-4 can direct isotype-specific class switching.
AB - We have characterized circular DNA in mouse splenocytes treated with the mitogen lipopolysaccharide (LPS) and various cytokines, including transforming growth factor β (TGF-β) and interleukin 4 (IL-4). Using probes of immunoglobulin heavy chain constant genes (CH), excision products of class switch recombination were identified. The majority of the clones contained the 3′ portion of the switch μ (Sμ) region and the 5′ portion of other switch regions. Some clones contained 3′-Sγ sequences instead of 3′-Sμ. This indicates that isotype switching may occur not only from Cμ, but also from one of the Cγ genes to other CH genes further downstream. In the presence of LPS, the cytokine TGF-β enhanced the detection of 5′-Sα-positive clones, while the lymphokine IL-4 enhanced 5′-Sγ1 positives. The data support the notion that TGF-β and IL-4 can direct isotype-specific class switching.
UR - http://www.scopus.com/inward/record.url?scp=0025369706&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025369706&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(90)90247-C
DO - 10.1016/0092-8674(90)90247-C
M3 - Article
C2 - 2114219
AN - SCOPUS:0025369706
SN - 0092-8674
VL - 62
SP - 135
EP - 142
JO - Cell
JF - Cell
IS - 1
ER -