Synaptic vesicle membrane fusion complex: Action of clostridial neurotoxins on assembly

Tetsuya Hayashi, Harvey McMahon, Shinji Yamasaki, Thomas Binz, Yutaka Hata, Thomas C. Südhof, Heiner Niemann

研究成果: ジャーナルへの寄稿記事

630 引用 (Scopus)

抜粋

Clostridial neurotoxins inhibit neurotransmitter release by selective and specific intracellular proteolysis of synaptobrevin/VAMP, synaptosomal-associated protein of 25 kDa (SNAP-25) or syntaxin. Here we show that in binary reactions synaptobrevin binds weakly to both SNAP-25 and syntaxin, and SNAP-25 binds to syntaxin. In the presence of all three components, a dramatic increase in the interaction strengths occurs and a stable sodium dodecyl sulfate-resistant complex forms. Mapping of the interacting sequences reveals that complex formation correlates with the presence of predicted α-helical structures, suggesting that membrane fusion involves intermolecular interactions via coiled-coil structures. Most toxins only attack the free, and not the complexed, proteins, and proteolysis of the proteins by different clostridial neurotoxins has distinct inhibitory effects on the formation of synaptobrevin-syntaxin-SNAP-25 complexes. Our data suggest that synaptobrevin, syntaxin and SNAP-25 associate into a unique stable complex that functions in synaptic vesicle exocytosis.

元の言語英語
ページ(範囲)5051-5061
ページ数11
ジャーナルEMBO Journal
13
発行部数21
DOI
出版物ステータス出版済み - 1 1 1994

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

これを引用

Hayashi, T., McMahon, H., Yamasaki, S., Binz, T., Hata, Y., Südhof, T. C., & Niemann, H. (1994). Synaptic vesicle membrane fusion complex: Action of clostridial neurotoxins on assembly. EMBO Journal, 13(21), 5051-5061. https://doi.org/10.1002/j.1460-2075.1994.tb06834.x