Synergistic antiproliferative effects of zoledronic acid and fluvastatin on human pancreatic cancer cell lines

An in Vitro study

Mahitab Elsayed, Daisuke Kobayashi, Toshio Kubota, Matsunaga Naoya, Ryusei Murata, Yuko Yoshizawa, Natsuki Watanabe, Tohru Matsuura, Yuya Tsurudome, Takashi Ogino, Shigehiro Ohdo, Takao Shimazoe

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

Bisphosphonates and statins are known to have antitumor activities against different types of cancer cell lines. In the present study, we investigated the antiproliferative effects of the combination of zoledronic acid (ZOL), a bisphophosphonate, and fluvastatin (FLU), a statin, in vitro on two types of human pancreatic cancer cell lines, Mia PaCa-2 and Suit-2. The pancreatic cancer cell lines were treated with ZOL and FLU both individually and in combination to evaluate their antiproliferative effects using WST-8 cell proliferation assay. In this study, we demonstrated a potent synergistic antiproliferative effect of both drugs when used in combination in both cell lines. Moreover, we studied the molecular mechanism behind this synergistic effect, which was inhibited by the addition of the mevalonate pathway products, farnesyl pyrophosphate (FPP) and geranylgeranyl pyrophosphate (GGPP). Furthermore, we aimed to determine the effect of ZOL and FLU combination on RhoA and Ras guanosine 5′-triphosphate (GTP)-proteins. The combination induced a marked accumulation in RhoA and unprenylated Ras. GGPP and FPP reversed the increase in the amount of both proteins. These results indicated that the combination treatment impaired RhoA and Ras signaling pathway by the inhibition of geranylgeranylation and/or farnesylation. This study provides a potentially effective approach for the treatment of pancreatic cancer using a combination treatment of ZOL and FLU.

元の言語英語
ページ(範囲)1238-1246
ページ数9
ジャーナルBiological and Pharmaceutical Bulletin
39
発行部数8
DOI
出版物ステータス出版済み - 1 1 2016

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zoledronic acid
fluvastatin
Pancreatic Neoplasms
Prenylation
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Cell Line
Mevalonic Acid
Diphosphonates
Guanosine Triphosphate
Proteins
Cell Proliferation
In Vitro Techniques
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

これを引用

Synergistic antiproliferative effects of zoledronic acid and fluvastatin on human pancreatic cancer cell lines : An in Vitro study. / Elsayed, Mahitab; Kobayashi, Daisuke; Kubota, Toshio; Naoya, Matsunaga; Murata, Ryusei; Yoshizawa, Yuko; Watanabe, Natsuki; Matsuura, Tohru; Tsurudome, Yuya; Ogino, Takashi; Ohdo, Shigehiro; Shimazoe, Takao.

:: Biological and Pharmaceutical Bulletin, 巻 39, 番号 8, 01.01.2016, p. 1238-1246.

研究成果: ジャーナルへの寄稿記事

Elsayed, Mahitab ; Kobayashi, Daisuke ; Kubota, Toshio ; Naoya, Matsunaga ; Murata, Ryusei ; Yoshizawa, Yuko ; Watanabe, Natsuki ; Matsuura, Tohru ; Tsurudome, Yuya ; Ogino, Takashi ; Ohdo, Shigehiro ; Shimazoe, Takao. / Synergistic antiproliferative effects of zoledronic acid and fluvastatin on human pancreatic cancer cell lines : An in Vitro study. :: Biological and Pharmaceutical Bulletin. 2016 ; 巻 39, 番号 8. pp. 1238-1246.
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T2 - An in Vitro study

AU - Elsayed, Mahitab

AU - Kobayashi, Daisuke

AU - Kubota, Toshio

AU - Naoya, Matsunaga

AU - Murata, Ryusei

AU - Yoshizawa, Yuko

AU - Watanabe, Natsuki

AU - Matsuura, Tohru

AU - Tsurudome, Yuya

AU - Ogino, Takashi

AU - Ohdo, Shigehiro

AU - Shimazoe, Takao

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AB - Bisphosphonates and statins are known to have antitumor activities against different types of cancer cell lines. In the present study, we investigated the antiproliferative effects of the combination of zoledronic acid (ZOL), a bisphophosphonate, and fluvastatin (FLU), a statin, in vitro on two types of human pancreatic cancer cell lines, Mia PaCa-2 and Suit-2. The pancreatic cancer cell lines were treated with ZOL and FLU both individually and in combination to evaluate their antiproliferative effects using WST-8 cell proliferation assay. In this study, we demonstrated a potent synergistic antiproliferative effect of both drugs when used in combination in both cell lines. Moreover, we studied the molecular mechanism behind this synergistic effect, which was inhibited by the addition of the mevalonate pathway products, farnesyl pyrophosphate (FPP) and geranylgeranyl pyrophosphate (GGPP). Furthermore, we aimed to determine the effect of ZOL and FLU combination on RhoA and Ras guanosine 5′-triphosphate (GTP)-proteins. The combination induced a marked accumulation in RhoA and unprenylated Ras. GGPP and FPP reversed the increase in the amount of both proteins. These results indicated that the combination treatment impaired RhoA and Ras signaling pathway by the inhibition of geranylgeranylation and/or farnesylation. This study provides a potentially effective approach for the treatment of pancreatic cancer using a combination treatment of ZOL and FLU.

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