Synergistic degradation of arabinoxylan by free and immobilized xylanases and arabinofuranosidase

Lili Jia, Geisa A.L.G. Budinova, Yusaku Takasugi, Shuhei Noda, Tsutomu Tanaka, Hirofumi Ichinose, Masahiro Goto, Noriho Kamiya

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

Effective degradation of hemicellulose is of utmost importance in a wide variety of applications in bioindustry. Five endoxylanases from different glycoside hydrolase families and microorganisms were tested with an arabinofuranosidase, Araf51A, for the hydrolysis of insoluble wheat arabinoxylan, which is a structural component of hemicellulose. The optimized combination was XynZ/Xyn11A/Araf51A with a loading ratio of 2:2:1, and the value of degree of synergy increased with the increase of Araf51A proportion in the enzyme mixture. Afterwards, selected enzymes were immobilized on commercial magnetic nanoparticles through covalent bonding. Both free and immobilized enzymes showed a similar conversion to reducing sugars after hydrolysis for 48 h. After 10 cycles, approximately 20% of the initial enzymatic activity of both the individual or mixture of immobilized enzymes was retained. A 5.5-fold increase in the production of sugars was obtained with a mixture of enzymes immobilized after 10 cycles in total compared with free enzymes. Importantly, a sustainable synergism between immobilized arabinofuranosidase and immobilized endoxylanases in the hydrolysis of arabinoxylan was demonstrated.

元の言語英語
ページ(範囲)268-275
ページ数8
ジャーナルBiochemical Engineering Journal
114
DOI
出版物ステータス出版済み - 10 15 2016

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Immobilized Enzymes
Endo-1,4-beta Xylanases
Hydrolysis
Enzymes
Sugars
Degradation
Glycoside Hydrolases
Microorganisms
Nanoparticles
Triticum
arabinoxylan
hemicellulose

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

これを引用

Synergistic degradation of arabinoxylan by free and immobilized xylanases and arabinofuranosidase. / Jia, Lili; Budinova, Geisa A.L.G.; Takasugi, Yusaku; Noda, Shuhei; Tanaka, Tsutomu; Ichinose, Hirofumi; Goto, Masahiro; Kamiya, Noriho.

:: Biochemical Engineering Journal, 巻 114, 15.10.2016, p. 268-275.

研究成果: ジャーナルへの寄稿記事

Jia, Lili ; Budinova, Geisa A.L.G. ; Takasugi, Yusaku ; Noda, Shuhei ; Tanaka, Tsutomu ; Ichinose, Hirofumi ; Goto, Masahiro ; Kamiya, Noriho. / Synergistic degradation of arabinoxylan by free and immobilized xylanases and arabinofuranosidase. :: Biochemical Engineering Journal. 2016 ; 巻 114. pp. 268-275.
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AU - Noda, Shuhei

AU - Tanaka, Tsutomu

AU - Ichinose, Hirofumi

AU - Goto, Masahiro

AU - Kamiya, Noriho

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