Synthesis and biological evaluation of a monocyclic Fc-binding antibody-recruiting molecule for cancer immunotherapy

Koichi Sasaki, Kyohei Muguruma, Rento Osawa, Akane Fukuda, Atsuhiko Taniguchi, Akihiro Kishimura, Yoshio Hayashi, Takeshi Mori, Yoshiki Katayama

研究成果: ジャーナルへの寄稿学術誌査読

抄録

Antibody-recruiting molecules (ARMs) are bispecific molecules composed of an antibody-binding motif and a target-binding motif that redirect endogenous antibodies to target cells to elicit immune responses. To enhance the translational potential of ARMs, it is crucial to design antibody/target-binding motifs that have strong affinity and are easy to synthesize. Here, we synthesized a novel Fc-binding ARM (Fc-ARM) that targets folate receptor (FR)-positive cancer cells, Reo-3, using a recently developed monocyclic peptide 15-Lys8Leu, which binds strongly to the Fc region of an antibody. Reo-3 bound to the Fc region of the antibody with aKdof 5.8 nM, and recruited a clinically used antibody mixture to attack FR-positive IGROV-1 cells as efficiently as Fc-ARM2, in which a bicyclic Fc-binding peptide was used. These results indicate that 15-Lys8Leu, which can be synthesized readily, is suitable for various applications including the development of Fc-ARMs.

本文言語英語
ページ(範囲)406-409
ページ数4
ジャーナルRSC Medicinal Chemistry
12
3
DOI
出版ステータス出版済み - 3月 2021

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子医療
  • 薬理学
  • 薬科学
  • 創薬
  • 有機化学

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