Synthesis and Electron Paramagnetic Resonance Studies of Oligodeoxynucleotides Containing 2-N-tert-Butylaminoxyl-2′-deoxyadenosines

Manami Kurita, Yoshitaka Higuchi, J. W. Mirc, Shintaro Matsumoto, Kazuteru Usui, Hiroshi Suemune, Mariko Aso

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

Oligodeoxynucleotides (ODNs) containing 2-N-tert-butylaminoxyl-2′-deoxyadenosine (A*) residues were synthesized to allow accurate monitoring of adenine motion by EPR spectroscopy through the agency of direct linkage of the acyclic aminoxyl group to the nucleobase, and EPR studies of the ODNs in single- and double-stranded forms were performed. Upon duplex formation, peak broadening and decreases in peak height were observed in EPR spectra, and the synthesized ODNs were shown to be excellent monitors of hybridization. Comparison of peak height and the h1/h0 signal ratio provided information on the relative mobility of A* in duplexes with different stability. A second set of ODNs each containing two A* residues at different intervals and four dA residues were also synthesized. For these ODNs, correlations were observed between the EPR spectral shapes of the duplexes and the number of dA residues between A* residues, thus demonstrating the potential of A* residues in monitoring of the structures of nucleic acids.

元の言語英語
ページ(範囲)2346-2352
ページ数7
ジャーナルChemBioChem
17
発行部数24
DOI
出版物ステータス出版済み - 12 14 2016

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Oligodeoxyribonucleotides
Electron Spin Resonance Spectroscopy
Paramagnetic resonance
Monitoring
Adenine
Nucleic Acids
Spectrum Analysis
2-N-tert-butylaminoxyl-2'-deoxyadenosine
Spectroscopy

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

これを引用

Synthesis and Electron Paramagnetic Resonance Studies of Oligodeoxynucleotides Containing 2-N-tert-Butylaminoxyl-2′-deoxyadenosines. / Kurita, Manami; Higuchi, Yoshitaka; Mirc, J. W.; Matsumoto, Shintaro; Usui, Kazuteru; Suemune, Hiroshi; Aso, Mariko.

:: ChemBioChem, 巻 17, 番号 24, 14.12.2016, p. 2346-2352.

研究成果: ジャーナルへの寄稿記事

Kurita, Manami ; Higuchi, Yoshitaka ; Mirc, J. W. ; Matsumoto, Shintaro ; Usui, Kazuteru ; Suemune, Hiroshi ; Aso, Mariko. / Synthesis and Electron Paramagnetic Resonance Studies of Oligodeoxynucleotides Containing 2-N-tert-Butylaminoxyl-2′-deoxyadenosines. :: ChemBioChem. 2016 ; 巻 17, 番号 24. pp. 2346-2352.
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abstract = "Oligodeoxynucleotides (ODNs) containing 2-N-tert-butylaminoxyl-2′-deoxyadenosine (A*) residues were synthesized to allow accurate monitoring of adenine motion by EPR spectroscopy through the agency of direct linkage of the acyclic aminoxyl group to the nucleobase, and EPR studies of the ODNs in single- and double-stranded forms were performed. Upon duplex formation, peak broadening and decreases in peak height were observed in EPR spectra, and the synthesized ODNs were shown to be excellent monitors of hybridization. Comparison of peak height and the h1/h0 signal ratio provided information on the relative mobility of A* in duplexes with different stability. A second set of ODNs each containing two A* residues at different intervals and four dA residues were also synthesized. For these ODNs, correlations were observed between the EPR spectral shapes of the duplexes and the number of dA residues between A* residues, thus demonstrating the potential of A* residues in monitoring of the structures of nucleic acids.",
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AU - Kurita, Manami

AU - Higuchi, Yoshitaka

AU - Mirc, J. W.

AU - Matsumoto, Shintaro

AU - Usui, Kazuteru

AU - Suemune, Hiroshi

AU - Aso, Mariko

PY - 2016/12/14

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N2 - Oligodeoxynucleotides (ODNs) containing 2-N-tert-butylaminoxyl-2′-deoxyadenosine (A*) residues were synthesized to allow accurate monitoring of adenine motion by EPR spectroscopy through the agency of direct linkage of the acyclic aminoxyl group to the nucleobase, and EPR studies of the ODNs in single- and double-stranded forms were performed. Upon duplex formation, peak broadening and decreases in peak height were observed in EPR spectra, and the synthesized ODNs were shown to be excellent monitors of hybridization. Comparison of peak height and the h1/h0 signal ratio provided information on the relative mobility of A* in duplexes with different stability. A second set of ODNs each containing two A* residues at different intervals and four dA residues were also synthesized. For these ODNs, correlations were observed between the EPR spectral shapes of the duplexes and the number of dA residues between A* residues, thus demonstrating the potential of A* residues in monitoring of the structures of nucleic acids.

AB - Oligodeoxynucleotides (ODNs) containing 2-N-tert-butylaminoxyl-2′-deoxyadenosine (A*) residues were synthesized to allow accurate monitoring of adenine motion by EPR spectroscopy through the agency of direct linkage of the acyclic aminoxyl group to the nucleobase, and EPR studies of the ODNs in single- and double-stranded forms were performed. Upon duplex formation, peak broadening and decreases in peak height were observed in EPR spectra, and the synthesized ODNs were shown to be excellent monitors of hybridization. Comparison of peak height and the h1/h0 signal ratio provided information on the relative mobility of A* in duplexes with different stability. A second set of ODNs each containing two A* residues at different intervals and four dA residues were also synthesized. For these ODNs, correlations were observed between the EPR spectral shapes of the duplexes and the number of dA residues between A* residues, thus demonstrating the potential of A* residues in monitoring of the structures of nucleic acids.

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