Synthesis and in vitro cell compatibility of α-tricalcium phosphate-based apatite cement containing tricalcium silicate

L. J. Cardenas, A. Takeuchi, Kanji Tsuru, S. Matsuya, Kunio Ishikawa

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

2 被引用数 (Scopus)

抄録

The presence of silicate in artificial bone graft material is known to be effective in increasing bone formation rate. We previously reported the basic setting properties of α-tricalcium phosphate (α-Ca 3PO4; α-TCP) based apatite cement (AC) with various amount of tricalcium silicate (Ca3SiO5; alite) addition using sodium dihydrogen phosphate (NaH2PO4) as the liquid phase. In this study, in vitro biological compatibility of pure α-TCP and 2.5-10.0 wt% alite added to α-TCP based AC is investigated in terms of cell attachment, proliferation and differentiation. Set ACs aged for 168 hrs were used for cell studies with bone marrow cells from tibia of 4 week-old, male, SD rat. Initial cell attachment was observed after 7 hrs; cell proliferation was observed after 3, 5, and 7 days. Cell differentiation (alkaline phosphatase activity) was observed after 3, 6, and 9 days of incubation. Cell attachment of alite added ACs were almost the same as sintered hydroxyapatite (HAp) and alite-free AC. Cell proliferation at 2.5-7.5 wt% alite added ACs were significantly higher at day 5 and 7 (p<0.05). Alkaline phosphatase activity revealed that 2.5 wt% alite addition accelerated the osteogenic cell activity at day 6 and 9 (p<0.05) whereas too much alite addition resulted in the decrease of osteogenic cell activity. In conclusion, we found that α-TCP based AC containing appropriate amount of alite could be an improved AC since it shows suitable setting time, higher mechanical strength and possible higher osteoconductivity.

本文言語英語
ホスト出版物のタイトルThe Third International Conference on the Development of Biomedical Engineering in Vietnam
ページ207-210
ページ数4
DOI
出版ステータス出版済み - 5 21 2010
イベント3rd International Conference on the Development of Biomedical Engineering in Vietnam - Ho Chi Minh City, ベトナム
継続期間: 1 11 20101 14 2010

出版物シリーズ

名前IFMBE Proceedings
27
ISSN(印刷版)1680-0737

その他

その他3rd International Conference on the Development of Biomedical Engineering in Vietnam
Countryベトナム
CityHo Chi Minh City
Period1/11/101/14/10

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering

フィンガープリント 「Synthesis and in vitro cell compatibility of α-tricalcium phosphate-based apatite cement containing tricalcium silicate」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル