Synthesis of fluorescent molecular probes based on cis-cinnamic acid and molecular imaging of lettuce roots

Hiroshi Fukuda, Keisuke Nishikawa, Yukihiro Fukunaga, Katsuhiro Okuda, Kozue Kodama, Kenji Matsumoto, Arihiro Kano, Mitsuru Shindo

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We synthesized azo dye- and fluorescence-labeled cis-cinnamic acid analogues possessing inhibitory activity against lettuce root growth and a trans-isomer without bioactivity as a control probe. The radicles incubated with the azo dye-labeled analogue were stained red, with their tips especially deeply dyed. The fluorescent images of the radicles incubated with each of these molecular probes depicted that the root cap was fluorescence-stained. However, images of the control radicles prepared by staining with the trans-isomer fluorescent probe did not show emission at the root cap. These contrasts suggest specific localization of the cis-cinnamate analogue at the columella cells.

元の言語英語
ページ(範囲)6492-6498
ページ数7
ジャーナルTetrahedron
72
発行部数41
DOI
出版物ステータス出版済み - 2016

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

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