Lithium titanium oxide (Li4Ti5O12) spinels are promising negative electrode materials for application in energy technology. In this work, we have synthesized Li4Ti5O 12 and investigated its structure, electronic properties, and electrochemical features using several analytical spectroscopy and microscopy-based techniques. The equally spaced fringe pattern indicated by the high-resolution transmission electron microscopy (HRTEM) along with electron diffraction revealed that the grown Li4Ti5O12 is well crystallized in the spinel structure without any indication of crystallographic defects such as dislocations or misfits. The electronic structure determination using high-resolution X-ray photoelectron spectroscopy (XPS) coupled with compositional studies using energy dispersive X-ray spectrometry (EDS) indicated the excellent chemical quality of the Li 4Ti5O12. Under the optimal synthetic condition, the sample delivers a discharge capacity of 161 mAh/g at C/12. The good cyclability of Li4Ti5O12 is attributed to the small expansion (ΔV≈%) of the elementary unit-cell.